ArticleThe Plant cell2015
Job Sharing in the Endomembrane System: Vacuolar Acidification Requires the Combined Activity of V-ATPase and V-PPase.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Insights into Salinity Stress-Induced Morpho-Physiological and Molecular Responses and Nanoparticle- and Nanobiochar-Mediated Tolerance Mechanisms During Seed Germination.Nanomaterials (Basel, Switzerland) · 2026Review
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Guard cell starch and malate metabolism facilitate stomatal opening in response to low COThe New phytologist · 2026Article
- Malate metabolism in horticultural crops: mechanistic insights and agricultural practices for quality improvement.Molecular horticulture · 2025Review
- Salicylic acid inhibits V-ATPase activity and restricts cell elongation.Plant physiology · 2025Article
- Revealing the glutamate switch: Insights into proton translocation in plant HBiophysical journal · 2025Article
- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- Green Synthesized Titanium Oxide Nanoparticles Promote Salt Tolerance in Soybean.International journal of molecular sciences · 2025Article
- Cellular pH homeostasis shapes root system architecture by modulating auxin-mediated developmental responses.Plant physiology · 2025Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- Modulation of morphogenesis and metabolism by plant cell biomechanics: from model plants to traditional herbs.Horticulture research · 2025Article
- UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis.Plant physiology · 2025Article
- ATG8ylation of vacuolar membrane protects plants against cell wall damage.Nature plants · 2025Article
- The fate and function of theFrontiers in plant science · 2025Article
- The Role of Female and Male Genes in Regulating Pollen Tube Guidance in Flowering Plants.Genes · 2024Review
- Discovery of a second-site nia2 mutation in the background of multiple Arabidopsis PIF-related mutants containing the pif3-3 allele.The New phytologist · 2024Article
- Overexpression of vacuolar HFrontiers in plant science · 2024Article
- The V-type ATPase enhances photosynthesis in marine phytoplankton and further links phagocytosis to symbiogenesis.Current biology : CB · 2023Article
- Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers.Horticulture research · 2023Article
- Roles of type II HFrontiers in plant science · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of a large central vacuole is one of the hallmarks of a prototypical plant cell, and the multiple functions of this compartment require massive fluxes of molecules across its limiting membrane, the tonoplast. Transport is assumed to be energized by the membrane potential and the proton gradient established by the combined activity of two proton pumps, the vacuolar H(+)-pyrophosphatase (V-PPase) and the vacuolar H(+)-ATPase (V-ATPase). Exactly how labor is divided between these two enzymes has remained elusive. Here, we provide evidence using gain- and loss-of-function approaches that lack of the V-ATPase cannot be compensated for by increased V-PPase activity. Moreover, we show that increased V-ATPase activity during cold acclimation requires the presence of the V-PPase. Most importantly, we demonstrate that a mutant lacking both of these proton pumps is conditionally viable and retains significant vacuolar acidification, pointing to a so far undetected contribution of the trans-Golgi network/early endosome-localized V-ATPase to vacuolar pH.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.