ArticlePloS one2015
Unusually Large Number of Mutations in Asexually Reproducing Clonal Planarian Dugesia japonica.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Limitations of serial cloning in mammals.Nature communications · 2026Article
- Red Midge Larvae Are an Invertebrate Alternative Diet to Beef Liver for Planarian Husbandry.Biomolecules · 2025Article
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- The Multiscale Wisdom of the Body: Collective Intelligence as a Tractable Interface for Next-Generation Biomedicine.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Mutational profile of the regenerative process and de novo genome assembly of the planarian Schmidtea polychroa.Nucleic acids research · 2024Article
- Embryos assist morphogenesis of others through calcium and ATP signaling mechanisms in collective teratogen resistance.Nature communications · 2024Article
- Isolation of planarian viable cells using fluorescence-activated cell sorting for advancing single-cell transcriptome analysis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Article
- Differences in neurotoxic outcomes of organophosphorus pesticides revealedFrontiers in toxicology · 2022Article
- Collagen IV differentially regulates planarian stem cell potency and lineage progression.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Planarians recruit piRNAs for mRNA turnover in adult stem cells.Genes & development · 2019Article
- Outstanding intraindividual genetic diversity in fissiparous planarians (Dugesia, Platyhelminthes) with facultative sex.BMC evolutionary biology · 2019Article
- Planarian regeneration as a model of anatomical homeostasis: Recent progress in biophysical and computational approaches.Seminars in cell & developmental biology · 2019Review
- The bioelectric code: An ancient computational medium for dynamic control of growth and form.Bio Systems · 2018Review
- Article
- Girardia dorotocephala transcriptome sequence, assembly, and validation through characterization of piwi homologs and stem cell progeny markers.Developmental biology · 2018Article
- Draft genome ofZoological letters · 2018Article
- Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms.Molecular reproduction and development · 2017Article
- Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a CaBiochimica et biophysica acta. Molecular cell research · 2017Article
- Dataset for aData in brief · 2016Article
- Vertically- and horizontally-transmitted memories - the fading boundaries between regeneration and inheritance in planaria.Biology open · 2016Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established a laboratory clonal strain of freshwater planarian (Dugesia japonica) that was derived from a single individual and that continued to undergo autotomous asexual reproduction for more than 20 years, and we performed large-scale genome sequencing and transcriptome analysis on it. Despite the fact that a completely clonal strain of the planarian was used, an unusually large number of mutations were detected. To enable quantitative genetic analysis of such a unique organism, we developed a new model called the Reference Gene Model, and used it to conduct large-scale transcriptome analysis. The results revealed large numbers of mutations not only outside but also inside gene-coding regions. Non-synonymous SNPs were detected in 74% of the genes for which valid ORFs were predicted. Interestingly, the high-mutation genes, such as metabolism- and defense-related genes, were correlated with genes that were previously identified as diverse genes among different planarian species. Although a large number of amino acid substitutions were apparently accumulated during asexual reproduction over this long period of time, the planarian maintained normal body-shape, behaviors, and physiological functions. The results of the present study reveal a unique aspect of asexual reproduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.