ArticlePLoS genetics2015
Conserved piRNA Expression from a Distinct Set of piRNA Cluster Loci in Eutherian Mammals.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed.
- The Role of RNA Interactions with PIWI in Regulating Gametogenesis, Fertilization, and Early Development Across Species.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Lifestyles ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Gene model for the ortholog ofbioRxiv : the preprint server for biology · 2026Article
- Article
- Conserved host genes act as anchors for fast-evolving piRNA clusters via promoter hijacking.Research square · 2026Article
- Regulation of Small RNAs by Exercise and Their Role in Insulin Sensitivity.bioRxiv : the preprint server for biology · 2026Article
- Article
- Rapid evolution and comparative analysis of piRNA clusters inbioRxiv : the preprint server for biology · 2026Article
- Analysis of somatic piRNAs in the malaria mosquitoRNA biology · 2025Article
- Recurrent expansion and rapid evolution of the Drosophilid RNAi pathway in testis.bioRxiv : the preprint server for biology · 2025Article
- Gene model for the ortholog ofbioRxiv : the preprint server for biology · 2025Article
- Genetic polymorphisms lead to major, locus-specific, variation in piRNA production in mouse.The EMBO journal · 2025Article
- Paramutation-Like Behavior of Genic piRNA-Producing Loci inInternational journal of molecular sciences · 2025Article
- Traffic Jam activates the Flamenco piRNA cluster locus and the Piwi pathway to ensure transposon silencing and Drosophila fertility.Cell reports · 2025Article
- Evolutionary innovations in germline biology of placental mammals identified by transcriptomics of first-wave spermatogenesis in opossum.Developmental cell · 2025Article
- Cracking the code: how piRNA pathway shapes spermatogenesis and combats male infertility.Frontiers in cell and developmental biology · 2025Review
- Influence of aging and maternal protein restriction on PIWI-interacting RNA expression in the offspring rat ventral prostate.Scientific reports · 2024Article
- Enigmatic Pachytene PIWI-Interacting RNAs.Genome biology and evolution · 2024Review
- FlaHMM: unistrandNAR genomics and bioinformatics · 2024Article
- Rapid evolution of piRNA clusters in theGenome research · 2024Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
The Piwi pathway is deeply conserved amongst animals because one of its essential functions is to repress transposons. However, many Piwi-interacting RNAs (piRNAs) do not base-pair to transposons and remain mysterious in their targeting function. The sheer number of piRNA cluster (piC) loci in animal genomes and infrequent piRNA sequence conservation also present challenges in determining which piC loci are most important for development. To address this question, we determined the piRNA expression patterns of piC loci across a wide phylogenetic spectrum of animals, and reveal that most genic and intergenic piC loci evolve rapidly in their capacity to generate piRNAs, regardless of known transposon silencing function. Surprisingly, we also uncovered a distinct set of piC loci with piRNA expression conserved deeply in Eutherian mammals. We name these loci Eutherian-Conserved piRNA cluster (ECpiC) loci. Supporting the hypothesis that conservation of piRNA expression across ~100 million years of Eutherian evolution implies function, we determined that one ECpiC locus generates abundant piRNAs antisense to the STOX1 transcript, a gene clinically associated with preeclampsia. Furthermore, we confirmed reduced piRNAs in existing mouse mutations at ECpiC-Asb1 and -Cbl, which also display spermatogenic defects. The Asb1 mutant testes with strongly reduced Asb1 piRNAs also exhibit up-regulated gene expression profiles. These data indicate ECpiC loci may be specially adapted to support Eutherian reproduction.
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