ArticlePloS one2015
Spatial Variation of the Gut Microbiota in Broiler Chickens as Affected by Dietary Available Phosphorus and Assessed by T-RFLP Analysis and 454 Pyrosequencing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 67 citations in OpenAlex.
- Unlocking Phytate with Phytase: A Meta-Analytic View of Meat-Type Chicken Muscle Growth and Bone Mineralization Potential.Animals : an open access journal from MDPI · 2024Review
- Time series of chicken stool metagenomics and egg metabolomics in changing production systems: preliminary insights from a proof-of-concept.One health outlook · 2024Article
- How did antibiotic growth promoters increase growth and feed efficiency in poultry?Poultry science · 2024Article
- Red osier dogwood and its use in animal nutrition: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- A Comprehensive Study of the Microbiome, Resistome, and Physical and Chemical Characteristics of Chicken Waste from Intensive Farms.Biomolecules · 2022Article
- Microbiota Composition of Mucosa and Interactions between the Microbes of the Different Gut Segments Could Be a Factor to Modulate the Growth Rate of Broiler Chickens.Animals : an open access journal from MDPI · 2022Article
- Connecting gut microbiomes and short chain fatty acids with the serotonergic system and behavior inFrontiers in physiology · 2022Review
- Effects of grazing mixed-grass pastures on growth performance, immune responses, and intestinal microbiota in free-range Beijing-you chickens.Poultry science · 2021Article
- Article
- Lactic Acid Bacteria - A Promising Tool for Controlling ChickenFrontiers in microbiology · 2021Review
- Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods.Poultry science · 2020Article
- Changes in the diversity and composition of gut microbiota in pigeon squabs infected with Trichomonas gallinae.Scientific reports · 2020Article
- The Gut Microbial Architecture of Efficiency Traits in the Domestic Poultry Model Species Japanese Quail (G3 (Bethesda, Md.) · 2020Article
- Effects on the Ileal Microbiota of Phosphorus and Calcium Utilization, Bird Performance, and Gender in Japanese Quail.Animals : an open access journal from MDPI · 2020Article
- Identification of the Key Molecular Drivers of Phosphorus Utilization Based on Host miRNA-mRNA and Gut Microbiome Interactions.International journal of molecular sciences · 2020Article
- Short-chain fatty acids and ceca microbiota profiles in broilers and turkeys in response to diets supplemented with phytase at varying concentrations, with or without xylanase.Poultry science · 2020Article
- Dietary fiber and chicken microbiome interaction: Where will it lead to?Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Review
- Review
- Development of the Ileal Microbiota in Three Broiler Breeds.Frontiers in veterinary science · 2020Article
- Broilers divergently selected for digestibility differ for their digestive microbial ecosystems.PloS one · 2020Article
Corrections and comments
- Erratum issuedCorrection: Spatial Variation of the Gut Microbiota in Broiler Chickens as Affected by Dietary Available Phosphorus and Assessed by T-RFLP Analysis and 454 Pyrosequencing.2015 · Camarinha da Silva, Amelia [corrected to Carminha-Silva, Amelia]
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular fingerprinting and sequencing based techniques have been widely used to characterize microbial communities. Terminal restriction fragment length polymorphism (T-RFLP) and 454-pyrosequencing were used to determine the microorganisms present in the different sections of the chicken gastrointestinal tract (GIT) (crop, jejunum, ileum and caeca). Broilers fed with diets differing in phosphorous (P) and calcium (Ca) as well as in phytase levels were used to study the microbiota of the upper and lower part of the GIT. A database with terminal restriction fragments (T-RF) of the most important organism present in the different gastrointestinal sections was constructed. The analysis revealed a distinct microbial assemblage on each section. Regardless of the diet, crop, jejunum and ileum were mainly colonized by Lactobacillaceae, and caeca were the most diverse site. The correlation between Lactobacillus crispatus and L. reuteri was positive in the crop, but negative in the jejunum. In crop samples, higher P and Ca levels led to a shift in the abundance of L. reuteri and L. crispatus to L. salivarius and L. taiwanensis whereas in the ileum supplementation of phytase favored L. salivarius and L. taiwanensis but resulted in decreased abundance of L. crispatus. Both methods were correlating significantly, being T-RFLP a reliable fingerprinting method to rapidly analyze large numbers of samples in a cost-effective and rapid manner. Results are easy to interpret with no need of deep bioinformatics knowledge and can be integrated with taxonomic information.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.