Evidence map›Paper›PMID 26587842›Full record

ArticlePloS one2015

Investigating the Role of the Host Multidrug Resistance Associated Protein Transporter Family in Burkholderia cepacia Complex Pathogenicity Using a Caenorhabditis elegans Infection Model.

Pietro Tedesco, Marco Visone, Ermenegilda Parrilli, Maria Luisa Tutino, Elena Perrin, Isabel Maida, Renato Fani, Francesco Ballestriero, Radleigh Santos, Clemencia Pinilla and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Methodological tools to study species of the genus Burkholderia.Applied microbiology and biotechnology · 2021
    Review
  2. Article
  3. Evaluation ofBio-protocol · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 8 institutions in 3 countries.

Pietro TedescoInstitute of Protein Biochemistry, National Research Council, Via P. Castellino 111, I-80131, Naples, Italy.
Marco VisoneInstitute of Protein Biochemistry, National Research Council, Via P. Castellino 111, I-80131, Naples, Italy.
Ermenegilda ParrilliDepartment of Chemical Sciences and School of Biotechnological Sciences, University of Naples Federico II, Via Cintia, I-80126, Naples, Italy.
Maria Luisa TutinoDepartment of Chemical Sciences and School of Biotechnological Sciences, University of Naples Federico II, Via Cintia, I-80126, Naples, Italy.
Elena PerrinLaboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano, I-50019, Sesto Fiorentino, Florence, Italy.
Isabel MaidaLaboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano, I-50019, Sesto Fiorentino, Florence, Italy.
Renato FaniLaboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano, I-50019, Sesto Fiorentino, Florence, Italy.
Francesco BallestrieroSchool of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney, 2052, New South Wales, Australia.
Radleigh SantosTorrey Pines Institute of Molecular Studies, Port St. Lucie, FL, United States of America, and San Diego, CA, United States of America.
Clemencia PinillaTorrey Pines Institute of Molecular Studies, Port St. Lucie, FL, United States of America, and San Diego, CA, United States of America.
Elia Di SchiaviInstitute of Bioscience and BioResources, National Research Council, via P. Castellino 111, I-80131, Naples, Italy.
George TegosTorrey Pines Institute of Molecular Studies, Port St. Lucie, FL, United States of America, and San Diego, CA, United States of America.
Donatella de PascaleInstitute of Protein Biochemistry, National Research Council, Via P. Castellino 111, I-80131, Naples, Italy.
University of Florence · ITUniversity of Naples Federico II · ITTorrey Pines Institute For Molecular Studies · USHarvard University · USInstitute of Genetics and Biophysics · ITInstitute of Protein Biochemistry · ITNational Research Council · ITUNSW Sydney · AU

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
NIH HHS P40 OD010440
6 · The paper itself

Abstract

This study investigated the relationship between host efflux system of the non-vertebrate nematode Caenorhabditis elegans and Burkholderia cepacia complex (Bcc) strain virulence. This is the first comprehensive effort to profile host-transporters within the context of Bcc infection. With this aim, two different toxicity tests were performed: a slow killing assay that monitors mortality of the host by intestinal colonization and a fast killing assay that assesses production of toxins. A Virulence Ranking scheme was defined, that expressed the toxicity of the Bcc panel members, based on the percentage of surviving worms. According to this ranking the 18 Bcc strains were divided in 4 distinct groups. Only the Cystic Fibrosis isolated strains possessed profound nematode killing ability to accumulate in worms' intestines. For the transporter analysis a complete set of isogenic nematode single Multidrug Resistance associated Protein (MRP) efflux mutants and a number of efflux inhibitors were interrogated in the host toxicity assays. The Bcc pathogenicity profile of the 7 isogenic C. elegans MRP knock-out strains functionality was classified in two distinct groups. Disabling host transporters enhanced nematode mortality more than 50% in 5 out of 7 mutants when compared to wild type. In particular mrp-2 was the most susceptible phenotype with increased mortality for 13 out 18 Bcc strains, whereas mrp-3 and mrp-4 knock-outs had lower mortality rates, suggesting a different role in toxin-substrate recognition. The use of MRP efflux inhibitors in the assays resulted in substantially increased (>40% on average) mortality of wild-type worms.

Indexed as

AnimalsATP-Binding Cassette, Sub-Family C ProteinsBurkholderia cepaciaBurkholderia InfectionsCaenorhabditis elegansDisease Models, AnimalDrug Resistance, MultipleGene Knockout TechniquesHumansMultidrug Resistance-Associated Protein 2ABCC2 protein, humanATP-Binding Cassette, Sub-Family C ProteinsMultidrug Resistance-Associated Protein 2

Identifiers

PMID26587842
PMCPMC4654563
OpenAlexW2176500907

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.