Evidence map›Paper›PMID 26587560›Full record

ArticleData in brief2015

Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes.

Franziska A Thomas, Ilaria Visco, Zdeněk Petrášek, Fabian Heinemann, Petra Schwille

Abstract read
In one paragraph

Article in Data in brief, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Cholesterol in the cargo membrane amplifies tau inhibition of kinesin-1-based transport.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Franziska A ThomasMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Ilaria ViscoMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Zdeněk PetrášekMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany ; Graz University of Technology, Institute of Biotechnology and Biochemical Engineering, Petersgasse 10-12/I, A-8010 Graz, Austria.
Fabian HeinemannMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany ; Roche Diagnostics, Nonnenwald 2, D-82377 Penzberg, Germany.
Petra SchwilleMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, a new and versatile assay to determine the partitioning coefficient [Formula: see text] as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, "Introducing a fluorescence-based standard to quantify protein partitioning into membranes" [1]. Here, the well-characterized binding of hexahistidine-tag (His6) to NTA(Ni) was utilized. Complementarily, this data article reports the average diffusion coefficient [Formula: see text] of His6-tagged enhanced green fluorescent protein (eGFP-His6) and the fluorescent lipid analog ATTO-647N-DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants [Formula: see text] of the NTA(Ni)/eGFP-His6 system are reported. Further, a conversion between [Formula: see text] and [Formula: see text] is provided.

Identifiers

PMID26587560
PMCPMC4625048

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.