Evidence map›Paper›PMID 26587470›Full record

ArticleIranian journal of public health2015

The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil.

Si Zhang, Zhaolian Lu, Hongmei Liang, Ximei Fu, Yan Zhang, Xin Liu, Genshu Bao, Tao Jing, Xuan Wang, Meng Wang and 2 more

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of public health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Mechanism of the Passage ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 1 country.

Si ZhangPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China ; Maternal and Child Health Hospital of Fengtai District of Beijing City, Beijing, China.
Zhaolian LuPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Hongmei LiangPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Ximei FuPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Yan ZhangDept. of Anesthesiology, First Hospital, Lanzhou University, Gansu, China.
Xin LiuInstitute of Integrated Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Genshu BaoPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Tao JingPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Xuan WangPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China ; Suqian Hospital of Traditional Chinese Medicine, Jiangsu, China.
Meng WangPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China ; Tianjin First Center Hospital, Tianjin, China.
Julong WuPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China ; Shandong Center for Disease Control and Prevention, Shandong, China.
Gen ChenPathogens Biology Institute, School of Basic Medical Sciences, Lanzhou University, Gansu, China ; Key Lab of Preclinical Study for New Drugs of Gansu Province, Gansu, China.
Lanzhou University · CNGansu University of Traditional Chinese Medicine · CNBeijing Fengtai Hospital · CNFirst Hospital of Lanzhou University · CNShandong Center for Disease Control and Prevention · CNTianjin First Center Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background5-Fluorouracil could lead to a decline in fertility in Caenorhabditis elegans. The aim of this study was to describe the mechanisms underlying such an altered fertility phenotype and to illustrate the specific genes and pathways that are involved in the related phenotypic changes in C. elegans.

methodsWe isolated total RNA from the samples and used a new method called Digital Gene Expression (DGE), which can rapidly identify genes with altered transcript levels. The random genes were confirmed by real-time RT-PCR.

resultsWe analyzed the results of two methods to draw conclusions based on a comparison between C. elegans and other harmful parasites. Compared with controls, 1147 genes were up-regulated, and 1067 were down-regulated. Overall, 101 up-regulated genes had a log2 ratio higher than 8, whereas the log2 ratio of 141 down-regulated genes was higher than 8. After mapping to the reference database, 4 pathways were confirmed to be involved in this phenomenon, with statistically significant participation from 19 genes.

conclusionFor the first time, the transcript sequence of 5-Fu-treated worms and controls was detected. We found that 4 possible pathways, i.e., ECM-receptor interaction pathway, TGF-beta signaling pathway, Focal adhesion and Hypertrophic cardiomyopathy, may be involved in the number decline in the embryos of C. elegans. Specifically, the ECM-receptor interaction pathway and Focal adhesion may be very important pathways that alter the reproduction of C. elegans.

Indexed as

5-FluorouracilCaenorhabditis elegansDigital gene expression

Identifiers

PMID26587470
PMCPMC4645726
OpenAlexW2188392666

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.