ArticlePlant physiology2016
Identification of Arabidopsis GPAT9 (At5g60620) as an Essential Gene Involved in Triacylglycerol Biosynthesis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed, 191 citations in OpenAlex.
- Identification and Functional Characterization of the GPAT Gene Family in Regulating Oil Biosynthesis in Olive.Plants (Basel, Switzerland) · 2026Article
- Diacylglycerol enantiomer selectivity of diacylglycerol acyltransferases highlights metabolic specialization in triacylglycerol synthesis across the tree of life.Bioscience reports · 2026Article
- Lipid droplet proteome plasticity in plant evolution, growth and development, and response to stress.Journal of experimental botany · 2026Review
- Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026Review
- Genetic defects in the CDP-choline pathway for phosphatidylcholine biosynthesis cannot be transmitted to offspring via male gametophytes owing to interruption of autophagy-like processes required for pollen germination in Arabidopsis thaliana.Journal of plant research · 2026Article
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Genome-wide characterization and transcriptional profiling of the glycerol-3-phosphate acyltransferase (GPAT) family in barley reveals the role of HvGPAT1 in low nitrogen tolerance.BMC plant biology · 2026Article
- Genome-wide association study and transcriptomic analysis reveal new genes for unsaturated fatty acid contents in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- The Interplay Between Nurr1 and Mitochondrial Biogenesis: Implications for Neurodegenerative Therapy.Molecular neurobiology · 2026Review
- Genome-wide analysis of theFrontiers in plant science · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Arabidopsis root lipid droplets are hubs for membrane homeostasis under heat stress, and triterpenoid synthesis and storage.The New phytologist · 2026Article
- Complete replacement of Arabidopsis oil-producing enzymes with heterologous diacylglycerol acyltransferases.Plant physiology · 2025Article
- Transcriptome Analysis Reveals Metabolic Pathways and Key Genes Involved in Oleic Acid Formation of Sunflower (International journal of molecular sciences · 2025Article
- Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (Plants (Basel, Switzerland) · 2025Article
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Regulatory mechanism of carbohydrate metabolism pathways on oil biosynthesis of oil plantFrontiers in plant science · 2025Article
- Genome-Wide Identification of the GPAT Family inPlants (Basel, Switzerland) · 2024Article
- Unravelling the molecular regulation network of carbon metabolism and lipid metabolism during seed development in Akebia trifoliata via integrated multi-omics analysis.Scientific reports · 2024Article
- Cuticle deposition ceases during strawberry fruit development.BMC plant biology · 2024Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The first step in the biosynthesis of nearly all plant membrane phospholipids and storage triacylglycerols is catalyzed by a glycerol-3-phosphate acyltransferase (GPAT). The requirement for an endoplasmic reticulum (ER)-localized GPAT for both of these critical metabolic pathways was recognized more than 60 years ago. However, identification of the gene(s) encoding this GPAT activity has remained elusive. Here, we present the results of a series of in vivo, in vitro, and in silico experiments in Arabidopsis (Arabidopsis thaliana) designed to assign this essential function to AtGPAT9. This gene has been highly conserved throughout evolution and is largely present as a single copy in most plants, features consistent with essential housekeeping functions. A knockout mutant of AtGPAT9 demonstrates both male and female gametophytic lethality phenotypes, consistent with the role in essential membrane lipid synthesis. Significant expression of developing seed AtGPAT9 is required for wild-type levels of triacylglycerol accumulation, and the transcript level is directly correlated to the level of microsomal GPAT enzymatic activity in seeds. Finally, the AtGPAT9 protein interacts with other enzymes involved in ER glycerolipid biosynthesis, suggesting the possibility of ER-localized lipid biosynthetic complexes. Together, these results suggest that GPAT9 is the ER-localized GPAT enzyme responsible for plant membrane lipid and oil biosynthesis.
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