ArticleNucleic acids research2016
A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Ssrp governs germline development independent of the FACT complex in Drosophila spermatogenesis.Cell & bioscience · 2025Article
- Histone and N-terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle Tribolium castaneum.Insect molecular biology · 2025Article
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos.Nucleic acids research · 2021Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomal instability in Saccharomyces cerevisiae.Genetics · 2021Article
- Epigenetic Regulation of ALS and CMT: A Lesson fromInternational journal of molecular sciences · 2021Review
- Review
- Spt6 is a maintenance factor for centromeric CENP-A.Nature communications · 2020Article
- A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation.Nucleic acids research · 2020Article
- Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.Genetics · 2020Article
- Localization of Drosophila CENP-A to non-centromeric sites depends on the NuRD complex.Nucleic acids research · 2019Article
- A role of the Trx-G complex in Cid/CENP-A deposition at Drosophila melanogaster centromeres.Chromosoma · 2019Article
- Protein kinases in mitotic phosphorylation of budding yeast CENP-A.Current genetics · 2019Review
- Hat1 acetylates histone H4 and modulates the transcriptional program in Drosophila embryogenesis.Scientific reports · 2019Article
- Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading.Nucleic acids research · 2019Article
- De novo formation and epigenetic maintenance of centromere chromatin.Current opinion in cell biology · 2019Review
- Posttranslational modifications of CENP-A: marks of distinction.Chromosoma · 2018Review
- Posttranslational mechanisms controlling centromere function and assembly.Current opinion in cell biology · 2018Review
- RNA cytosine methyltransferase Nsun3 regulates embryonic stem cell differentiation by promoting mitochondrial activity.Cellular and molecular life sciences : CMLS · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The incorporation of CENP-A into centromeric chromatin is an essential prerequisite for kinetochore formation. Yet, the molecular mechanisms governing this process are surprisingly divergent in different organisms. While CENP-A loading mechanisms have been studied in some detail in mammals, there are still large gaps to our understanding of CENP-A/Cid loading pathways in Drosophila. Here, we report on the characterization and delineation of at least three different CENP-A preloading complexes in Drosophila. Two complexes contain the CENP-A chaperones CAL1, FACT and/or Caf1/Rbap48. Notably, we identified a novel complex consisting of the histone acetyltransferase Hat1, Caf1 and CENP-A/H4. We show that Hat1 is required for proper CENP-A loading into chromatin, since knock-down in S2 cells leads to reduced incorporation of newly synthesized CENP-A. In addition, we demonstrate that CENP-A/Cid interacts with the HAT1 complex via an N-terminal region, which is acetylated in cytoplasmic but not in nuclear CENP-A. Since Hat1 is not responsible for acetylation of CENP-A/Cid, these results suggest a histone acetyltransferase activity-independent escort function for Hat1. Thus, our results point toward intriguing analogies between the complex processing pathways of newly synthesized CENP-A and canonical histones.
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