Evidence map›Paper›PMID 26586621›Full record

ArticleFitoterapia2016

Hydroxycinnamic acid amides from Scopolia tangutica inhibit the activity of M1 muscarinic acetylcholine receptor in vitro.

Yan Zhang, Zhen Long, Zhimou Guo, Zhiwei Wang, Xiuli Zhang, Richard D Ye, Xinmiao Liang, Olivier Civelli

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fitoterapia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.1field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Yan ZhangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address: zhangyan_sjtu@sjtu.edu.cn.
Zhen LongKey laboratory of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
Zhimou GuoKey laboratory of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
Zhiwei WangDepartment of Pharmacology, University of California, Irvine, CA 92697, United States.
Xiuli ZhangKey laboratory of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
Richard D YeSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Xinmiao LiangKey laboratory of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
Olivier CivelliDepartment of Pharmacology, University of California, Irvine, CA 92697, United States.
Dalian Institute of Chemical Physics · CNShanghai Jiao Tong University · CNUniversity of California, Irvine · USChinese Academy of Sciences · CN

Funding

CHARACTERIZATION OF TWO NOVEL NEUROPEPTIDESR01MH060231 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI CIVELLI, OLIVIER · 1999 to 2008
$2.7M
Antioxidant polyphenols from fruits of the Myrtle familyF31AT000801 · NCCIH · HERBERT H. LEHMAN COLLEGE · PI REYNERTSON, KURT ALLERSLEV · 2001 to 2005
$152k
NCCIH NIH HHS AT00801NCCIH NIH HHS F31 AT000801NIMH NIH HHS MH60231NIMH NIH HHS R01 MH060231
6 · The paper itself

Abstract

Scopolia tangutica Maxim (S. tangutica) extracts have been traditionally used as antispasmodic, sedative, and analgesic agents in Tibet and in the Qinghai province of China. Their active compositions are however poorly understood. We have recently isolated five new hydroxycinnamic acid (HCA) amides along with two known HCA amides, one cinnamic acid amide from these extracts. In this study, we evaluate their abilities to inhibit carbacol-induced activity of M1 muscarinic acetylcholine receptor along with the crude extracts. Chinese hamster ovary cells stably expressing the recombinant human M1 receptor (CHO-M1 cells) were employed to evaluate the anticholinergic potentials. Intracellular Ca(2+) changes were monitored using the FLIPR system. Five HCA amides as well as the crude S. tangutica extract displayed dose-dependent inhibitory effects against M1 receptor. These findings demonstrate that HCA amides are part of the M1 receptor-inhibiting principles of S. tangutica. Since blockade of parasympathetic nerve impulse transmission through the inhibition of the M1 receptor lessens smooth muscle spasms, our findings provided a molecular explanation for the traditional use of S. tangutica against spasm.

Indexed as

AnimalsCHO CellsCoumaric AcidsCricetulusDrugs, Chinese HerbalHumansMolecular StructureMuscarinic AntagonistsPlant ExtractsPlant RootsReceptor, Muscarinic M1Recombinant ProteinsScopoliaCoumaric AcidsDrugs, Chinese HerbalMuscarinic AntagonistsPlant ExtractsReceptor, Muscarinic M1Recombinant ProteinsHydroxycinnamic acid amidesM1 muscarinic acetylcholine receptorScopolia tangutica Maxim

Identifiers

PMID26586621
PMCPMC4698058
OpenAlexW2172947207

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.