Evidence map›Paper›PMID 26586188›Full record

ArticleScience (New York, N.Y.)2015

High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.

Yiyang Gong, Cheng Huang, Jin Zhong Li, Benjamin F Grewe, Yanping Zhang, Stephan Eismann, Mark J Schnitzer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 264 papers.

0numbers the graph read from it
0cells of the map it votes in
264citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

264 citing papers in PubMed.

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204 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Yiyang GongJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. yiyang.gong@duke.edu mschnitz@stanford.edu.
Cheng HuangJames H. Clark Center, Stanford University, Stanford, CA 94305, USA.
Jin Zhong LiJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA.
Benjamin F GreweJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA.
Yanping ZhangJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Stephan EismannJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA.
Mark J SchnitzerJames H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA. yiyang.gong@duke.edu mschnitz@stanford.edu.

Funding

Massively Parallel Brain ImagingDP1OD003560 · OD · STANFORD UNIVERSITY · PI SCHNITZER, MARK J · 2007 to 2011
$3.9M
Protein voltage sensors: kilohertz imaging of neural dynamics in behaving animalsU01NS090600 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z., SCHNITZER, MARK J · 2014 to 2016
$2.8M
Howard Hughes Medical InstituteNIH HHS DP1 OD003560NINDS NIH HHS U01 NS090600
6 · The paper itself

Abstract

Genetically encoded voltage indicators (GEVIs) are a promising technology for fluorescence readout of millisecond-scale neuronal dynamics. Previous GEVIs had insufficient signaling speed and dynamic range to resolve action potentials in live animals. We coupled fast voltage-sensing domains from a rhodopsin protein to bright fluorophores through resonance energy transfer. The resulting GEVIs are sufficiently bright and fast to report neuronal action potentials and membrane voltage dynamics in awake mice and flies, resolving fast spike trains with 0.2-millisecond timing precision at spike detection error rates orders of magnitude better than previous GEVIs. In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation. These results empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.

Indexed as

Action PotentialsBioluminescence Resonance Energy Transfer TechniquesBiosensing TechniquesEvoked Potentials, SomatosensoryFluorescence Resonance Energy TransferAnimalsDendritesDrosophila melanogasterGreen Fluorescent ProteinsMiceNeuronsRecombinant Fusion ProteinsRhodopsinSmellGreen Fluorescent ProteinsRecombinant Fusion ProteinsRhodopsin

Identifiers

PMID26586188
PMCPMC4904846

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.