ArticleAsian journal of andrology
Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro.
Article in Asian journal of andrology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Human embryonic stem cell-derived Sertoli cells as an immune modulator of cell transplantation therapy in a diabetic mice model.Stem cell research & therapy · 2025Article
- Review
- Sertoli cells as a hub in testicular development and male reproductive.Frontiers in cell and developmental biology · 2025Review
- The Basic Requirement of Tight Junction Proteins in Blood-Brain Barrier Function and Their Role in Pathologies.International journal of molecular sciences · 2024Review
- Effect of Metformin on Sertoli Cell Fatty Acid Metabolism and Blood-Testis Barrier Formation.Biology · 2024Article
- Atopic Dermatitis: Fertility, Pregnancy, and Treatment Perspectives.American journal of clinical dermatology · 2024Review
- RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells.Cell & bioscience · 2023Article
- Transmigration of macrophages through primary adult rat Sertoli cells.Tissue barriers · 2023Article
- Effects of iron oxide nanoparticles as TJournal of nanobiotechnology · 2022Article
- Muscarinic acetylcholine receptor M5 is involved in spermatogenesis through the modification of cell-cell junctions.Reproduction (Cambridge, England) · 2021Article
- miR-122-5p regulates the tight junction of the blood-testis barrier of mice via occludin : miR-122-5p can regulate the tight junction.Basic and clinical andrology · 2021Article
- Article
- UXT in Sertoli cells is required for blood-testis barrier integrity†.Biology of reproduction · 2020Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- Development and function of smooth muscle cells is modulated byDevelopment (Cambridge, England) · 2020Article
- Review
- CAMSAP2 Is a Microtubule Minus-End Targeting Protein That Regulates BTB Dynamics Through Cytoskeletal Organization.Endocrinology · 2019Article
- Tight junction proteins at the blood-brain barrier: far more than claudin-5.Cellular and molecular life sciences : CMLS · 2019Article
- Gliclazide alone or in combination with atorvastatin ameliorated reproductive damage in streptozotocin-induced type 2 diabetic male rats.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2019Article
- A Human Gonadal Cell Model From Induced Pluripotent Stem Cells.Frontiers in genetics · 2018Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Sertoli cell tight junction (TJ) is the key component of the blood-testis barrier, where it sequesters developing germ cells undergoing spermatogenesis within the seminiferous tubules. Hormonally regulated claudin-11 is a critical transmembrane protein involved in barrier function and its murine knockout results in infertility. We aimed to assess quantitatively the significance of the contribution of claudin-11 to TJ function, in vitro, using siRNA-mediated gene silencing. We also conducted an analysis of the contribution of occludin, another intrinsic transmembrane protein of the TJ. Silencing of claudin-11 and/or occludin was conducted using siRNA in an immature rat Sertoli cell culture model. Transepithelial electrical resistance was used to assess quantitatively TJ function throughout the culture. Two days after siRNA treatment, cells were fixed for immunocytochemical localization of junction proteins or lyzed for RT-PCR assessment of mRNA expression. Silencing of claudin-11, occludin, or both resulted in significant decreases in TJ function of 55% (P < 0.01), 51% (P < 0.01), and 62% (P < 0.01), respectively. Data were concomitant with significant decreases in mRNA expression and marked reductions in the localization of targeted proteins to the Sertoli cell TJ. We provide quantitative evidence that claudin-11 contributes significantly (P < 0.01) to Sertoli cell TJ function in vitro. Interestingly, occludin, which is hormonally regulated but not implicated in infertility until late adulthood, is also a significant (P < 0.01) contributor to barrier function. Our data are consistent with in vivo studies that clearly demonstrate a role for these proteins in maintaining normal TJ barrier structure and function.
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