ArticleJournal of experimental botany2016
Antisense down-regulation of the strawberry β-galactosidase gene FaβGal4 increases cell wall galactose levels and reduces fruit softening.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 145 citations in OpenAlex.
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- QTL mapping for berry firmness and functional characterization of a prioritized candidate gene VvNAC83 in grape.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- FaRIF as a key regulator of strawberry fruit ripening: deciphering its targets and interaction networks.Horticulture research · 2026Article
- Article
- The MabZIP5-MaMYB69 module cooperates with MaERF55 to modulate banana fruit ripening via cell wall degradation.Horticulture research · 2026Article
- Role of biotechnology for shelf-life extension and quality improvement of perishable fruits and vegetables: a comprehensive review.Food science and biotechnology · 2025Review
- Advances in Cell Wall Dynamics and Gene Expression in Postharvest Fruit Softening.Plants (Basel, Switzerland) · 2025Review
- ERF100 regulated by ERF28 and NOR controls pectate lyase 7, modulating fig (Ficus carica L.) fruit softening.Plant biotechnology journal · 2025Article
- Integrated omics reveal the mechanisms underlying softening and aroma changes in pear during postharvest storage and the role of melatonin.BMC plant biology · 2025Article
- Enrichment of two important metabolites D-galacturonic acid and D-glucuronic acid inhibits MdHb1-mediated fruit softening in apple.Nature plants · 2025Article
- PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri.BMC genomics · 2025Article
- VvARF19 represses VvLBD13-mediated cell wall degradation to delay softening of grape berries.Horticulture research · 2025Article
- Loss-of-function mutations in the fruit softening geneHorticulture research · 2025Article
- Enhancing quality and climate resilient traits in vegetatively propagated polyploids: transgenic and genome editing advancements, challenges and future directions.Frontiers in genetics · 2025Review
- Combined transcriptomic and proteomic analysis reveals the response mechanisms of alfalfa to freezing stress.Frontiers in plant science · 2025Article
- Novel Targets for Fruit Conservation Strategies Revealed by Omics Studies: A Systematic Review and Meta-Analysis.International journal of food science · 2025Review
- The MaNAP1-MaMADS1 transcription factor module mediates ethylene-regulated peel softening and ripening in banana.The Plant cell · 2024Article
- Genome-Wide Association Study of Grape Texture Based on Puncture.International journal of molecular sciences · 2024Article
- Effect of Short-Term High-COPlants (Basel, Switzerland) · 2024Article
- AUXIN RESPONSE FACTOR 2 mediates repression of strawberry receptacle ripening via auxin-ABA interplay.Plant physiology · 2024Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strawberry softening is characterized by an increase in the solubilization and depolymerization of pectins from cell walls. Galactose release from pectin side chains by β-galactosidase enzymes has been proposed as one reason for the increase in soluble pectins. A putative β-galactosidase gene, FaβGal4, has been identified using a custom-made oligonucleotide-based strawberry microarray platform. FaβGal4 was expressed mainly in the receptacle during fruit ripening, and was positively regulated by abscisic acid and negatively regulated by auxins. To ascertain the role of FaβGal4 in strawberry softening, transgenic plants containing an antisense sequence of this gene under the control of the CaMV35S promoter were generated. Phenotypic analyses were carried out in transgenic plants during three consecutive growing seasons, using non-transformed plants as control. Two out of nine independent transgenic lines yielded fruits that were 30% firmer than control at the ripe stage. FaβGal4 mRNA levels were reduced by 70% in ripe fruits from these selected transgenic lines, but they also showed significant silencing of FaβGal1, although the genes did not share significant similarity. These two transgenic lines also showed an increase in pectin covalently bound to the cell wall, extracted using Na2CO3. The amount of galactose in cell walls from transgenic fruits was 30% higher than in control; notably, the galactose increase was larger in the 1 M KOH fraction, which is enriched in hemicellulose. These results suggest that FaβGal4 participates in the solubilization of covalently bound pectins during ripening, reducing strawberry fruit firmness.
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