ArticleMethods in molecular biology (Clifton, N.J.)2016
Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.ACS measurement science au · 2024Review
- Article
- Novel Isobaric Tagging Reagent Enabled Multiplex Quantitative Glycoproteomics via Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2022Article
- Phosphoproteomic Identification of Vasopressin/cAMP/Protein Kinase A-Dependent Signaling in Kidney.Molecular pharmacology · 2021Review
- TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis.Journal of visualized experiments : JoVE · 2020Article
- Developing Workflow for Simultaneous Analyses of Phosphopeptides and Glycopeptides.ACS chemical biology · 2019Article
- Protein Mass Spectrometry Made Simple.Journal of the American Society of Nephrology : JASN · 2018Article
- High-Resolution Mapping of RNA-Binding Regions in the Nuclear Proteome of Embryonic Stem Cells.Molecular cell · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Isobaric tagging reagents have become an invaluable tool for multiplexed quantitative proteomic analysis. These reagents can label multiple, distinct peptide samples from virtually any source material (e.g., tissue, cell line, purified proteins), allowing users the opportunity to assess changes in peptide abundances across many different time points or experimental conditions. Here, we describe the application of isobaric peptide labeling, specifically 8plex isobaric tags for relative and absolute quantitation (8plex iTRAQ), for quantitative phosphoproteomic analysis of cultured cells or tissue suspensions. For this particular protocol, labeled samples are pooled, fractionated by strong cation exchange chromatography, enriched for phosphopeptides, and analyzed by tandem mass spectrometry (LC-MS/MS) for both peptide identification and quantitation.
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Registered trials
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