Evidence map›Paper›PMID 26584544›Full record

ArticleStem cell reports2016

A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies.

Xiaoqing Zhu, Bo Li, Zongyong Ai, Zheng Xiang, Kunshang Zhang, Xiaoyan Qiu, Yongchang Chen, Yuemin Li, Joshua D Rizak, Yuyu Niu and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. Integrative Analysis of Key Signalling Pathways in Neural Tube Defects: From Molecular Mechanisms to Therapeutic Strategies.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2025
    Review
  4. Article
  5. Article
  6. Review
  7. Science advances · 2022
    Article
  8. Identification and functional characterization of CD133Molecular and cellular biochemistry · 2022
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xiaoqing ZhuYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China.
Bo LiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China.
Zongyong AiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China.
Zheng XiangYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China.
Kunshang ZhangTranslational Stem Cell Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China.
Xiaoyan QiuYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China.
Yongchang ChenYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China.
Yuemin LiChongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China.
Joshua D RizakKunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China.
Yuyu NiuYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China.
Xintian HuKunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China.
Yi Eve SunYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; Translational Stem Cell Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China.
Weizhi JiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China. Electronic address: wji@kbimed.com.
Tianqing LiYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China. Electronic address: litq@kbimed.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Developing a model of primate neural tube (NT) development is important to promote many NT disorder studies in model organisms. Here, we report a robust and stable system to allow for clonal expansion of single monkey neuroepithelial stem cells (NESCs) to develop into miniature NT-like structures. Single NESCs can produce functional neurons in vitro, survive, and extensively regenerate neuron axons in monkey brain. NT formation and NESC maintenance depend on high metabolism activity and Wnt signaling. NESCs are regionally restricted to a telencephalic fate. Moreover, single NESCs can turn into radial glial progenitors (RGPCs). The transition is accurately regulated by Wnt signaling through regulation of Notch signaling and adhesion molecules. Finally, using the "NESC-TO-NTs" system, we model the functions of folic acid (FA) on NT closure and demonstrate that FA can regulate multiple mechanisms to prevent NT defects. Our system is ideal for studying NT development and diseases.

Indexed as

AnimalsAnimals, NewbornCell Culture TechniquesCell LineageCell ProliferationClone CellsDisease Models, AnimalFolic Acid DeficiencyMacaca mulattaNeural TubeNeural Tube DefectsNeuroepithelial CellsNeurogliaNeuronsStem CellsTelencephalon

Identifiers

PMID26584544
PMCPMC4750068

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.