ArticleNature protocols2015
Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Dermal Dive: An Overview of Cutaneous Wounding Techniques in Zebrafish.The Journal of investigative dermatology · 2024Review
- Macrophages in tissue repair and regeneration: insights from zebrafish.Cell regeneration (London, England) · 2024Review
- Live Imaging of Cutaneous Wound Healing after Rotary Tool Injury in Zebrafish.The Journal of investigative dermatology · 2024Article
- Continuous anesthesia for 60 days in an isosmotic environment does not impair limb or cardiac regeneration in the axolotl.Scientific reports · 2023Article
- Temporally and regionally distinct morphogenetic processes govern zebrafish caudal fin blood vessel network expansion.Development (Cambridge, England) · 2023Article
- Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis.Disease models & mechanisms · 2023Article
- Longitudinal in vivo imaging of adult Danionella cerebrum using standard confocal microscopy.Disease models & mechanisms · 2022Article
- Long-term imaging of living adult zebrafish.Development (Cambridge, England) · 2022Article
- Microtubule organization of vertebrate sensory neurons in vivo.Developmental biology · 2021Article
- Live Imaging of Intracranial Lymphatics in the Zebrafish.Circulation research · 2021Article
- Can laboratory model systems instruct human limb regeneration?Development (Cambridge, England) · 2019Article
- Bacteria evoke alarm behaviour in zebrafish.Nature communications · 2019Article
- In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone.Current biology : CB · 2018Article
- Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue.Journal of visualized experiments : JoVE · 2018Article
- Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization.Developmental cell · 2018Article
- Article
- In vivo physiological recording from the lateral line of juvenile zebrafish.The Journal of physiology · 2016Article
- Single-cell in vivo imaging of adult neural stem cells in the zebrafish telencephalon.Nature protocols · 2016Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zebrafish possess the remarkable ability to regenerate a vast variety of tissues, even as adults. However, direct imaging of regenerative processes in adult zebrafish remains challenging because of the lack of suitable anesthesia protocols. Here we present a description of an intubation-based anesthesia procedure that we developed to enable us to image regenerating zebrafish fins and which can be used to continuously anesthetize adult zebrafish for up to 2 d. Fish are immobilized in an imaging chamber followed by oral intubation. Subsequent delivery of anesthetic-containing water is achieved via a peristaltic pump. The setup of the system will take ∼90 min for two adult zebrafish, and it requires only a little previous experience of working with zebrafish. Our protocol will enable the imaging of regenerative processes in the fin and other tissues, and the investigation of processes that require long-term anesthesia, such as immune responses and surgical procedures.
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Registered trials
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