ArticleNature protocols2015
Single-molecule fluorescence imaging to quantify membrane protein dynamics and oligomerization in living plant cells.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- The VAMP-associated protein VAP27-1 plays a crucial role in plant resistance to ER stress by modulating ER-PM contact architecture in Arabidopsis.Plant communications · 2024Article
- Arabidopsis HSFA9 Acts as a Regulator of Heat Response Gene Expression and the Acquisition of Thermotolerance and Seed Longevity.Plant & cell physiology · 2024Article
- VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells.Plant methods · 2023Article
- High Electrochemiluminescence from Ru(bpy)Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Spatially resolved single-molecule profiling of microRNAs in migrating cells driven by microconfinement.Chemical science · 2022Article
- Polyvinyl Chloride Nanoparticles Affect Cell Membrane Integrity by Disturbing the Properties of the Multicomponent Lipid Bilayer inMolecules (Basel, Switzerland) · 2022Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers.Nature methods · 2021Article
- Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.Membranes · 2021Article
- Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques.Science China. Life sciences · 2021Review
- Xanthomonas effector XopR hijacks host actin cytoskeleton via complex coacervation.Nature communications · 2021Article
- Multiple functions of the vacuole in plant growth and fruit quality.Molecular horticulture · 2021Review
- Single-Molecule Imaging in Living Plant Cells: A Methodological Review.International journal of molecular sciences · 2021Review
- In vivo single-particle tracking of the aquaporin AtPIP2;1 in stomata reveals cell type-specific dynamics.Plant physiology · 2021Article
- Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues.Nature protocols · 2021Article
- Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells.Nature protocols · 2021Article
- Visualising endocytosis in plants: past, present, and future.Journal of microscopy · 2020Article
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
- The continuing arc toward phototropic enlightenment.Journal of experimental botany · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring the mobility and interactions of proteins is key to understanding cellular signaling mechanisms; however, quantitative analysis of protein dynamics in living plant cells remains a major challenge. Here we describe an automated, single-molecule protocol based on total internal reflection fluorescence microscopy (TIRFM) imaging that allows protein tracking and subunit counting in living plant cells. This protocol uses TIRFM to image transgenic plant tissues expressing fluorescently tagged proteins that are localized to the plasma membrane. Next, a tracking algorithm quantifies dynamic changes in fluorescent protein motion types, temporary particle displacement and protein photobleaching steps. This protocol allows researchers to study the kinetic characteristics of heterogeneously distributed proteins. The approach has potential applications for studies of protein dynamics and subunit stoichiometry for a wide variety of plasma membrane and intracellular proteins in living plant cells and other biological specimens visualized by TIRFM or other fluorescence imaging techniques. The whole protocol can be completed in 5-6 h.
Indexed as
Identifiers
26584445What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.