Evidence map›Paper›PMID 26584444›Full record

ArticleNature protocols2015

Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens.

Sandra March, Vyas Ramanan, Kartik Trehan, Shengyong Ng, Ani Galstian, Nil Gural, Margaret A Scull, Amir Shlomai, Maria M Mota, Heather E Fleming and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed.

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  18. Supernatant of platelet-Microbiology spectrum · 2024
    Article
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27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sandra MarchBroad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Vyas RamananDepartment of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Kartik TrehanDepartment of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Shengyong NgDepartment of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Ani GalstianBroad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
Nil GuralDepartment of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Margaret A ScullLaboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, New York, USA.
Amir ShlomaiLaboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, New York, USA.
Maria M MotaUnidade de Malaria, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal.
Heather E FlemingDepartment of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Salman R KhetaniDepartment of Mechanical Engineering, School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado, USA.
Charles M RiceLaboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, New York, USA.
Sangeeta N BhatiaBroad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Funding

Modeling human hepatotropic infections in complex tissue organoidsR01DK085713 · NIDDK · ROCKEFELLER UNIVERSITY · PI BHATIA, SANGEETA N., RICE, CHARLES M · 2009 to 2013
$5.6M
Howard Hughes Medical InstituteNIDDK NIH HHS R01 DK085713NIDDK NIH HHS R01 DK85713
6 · The paper itself

Abstract

The development of therapies and vaccines for human hepatropic pathogens requires robust model systems that enable the study of host-pathogen interactions. However, in vitro liver models of infection typically use either hepatoma cell lines that exhibit aberrant physiology or primary human hepatocytes in culture conditions in which they rapidly lose their hepatic phenotype. To achieve stable and robust in vitro primary human hepatocyte models, we developed micropatterned cocultures (MPCCs), which consist of primary human hepatocytes organized into 2D islands that are surrounded by supportive fibroblast cells. By using this system, which can be established over a period of days, and maintained over multiple weeks, we demonstrate how to recapitulate in vitro hepatic life cycles for the hepatitis B and C viruses and the Plasmodium pathogens P. falciparum and P. vivax. The MPCC platform can be used to uncover aspects of host-pathogen interactions, and it has the potential to be used for drug and vaccine development.

Indexed as

Host-Pathogen InteractionsAnimalsCell LineCells, CulturedCoculture TechniquesFibroblastsHepacivirusHepatitis BHepatitis B virusHepatitis CHepatocytesHumansMalariaMicePlasmodiumPlasmodium falciparum

Identifiers

PMID26584444
PMCPMC5867906

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.