ArticlePLoS neglected tropical diseases2015
Simple, Rapid Mycobacterium ulcerans Disease Diagnosis from Clinical Samples by Fluorescence of Mycolactone on Thin Layer Chromatography.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Field deployment of a mobile suitcase laboratory for Buruli ulcer diagnosis in Ghana.PLoS neglected tropical diseases · 2026Article
- Suspected Buruli ulcer cases in Tonkolili District, Sierra Leone- a prospective cohort study.Infection · 2025Article
- Review
- Evaluation of an electricity-independent method for IS2404 Loop-mediated isothermal amplification (LAMP) diagnosis of Buruli ulcer in resource-limited settings.PLoS neglected tropical diseases · 2024Article
- A single synthetic lipid antigen for improved serological diagnosis of Buruli ulcer.Public health action · 2023Article
- Alternative boronic acids in the detection of Mycolactone A/B using the thin layer chromatography (f-TLC) method for diagnosis of Buruli ulcer.BMC infectious diseases · 2023Article
- Evaluation of the fluorescent-thin layer chromatography (f-TLC) for the diagnosis of Buruli ulcer disease in Ghana.PloS one · 2022Article
- Novel Diagnostics for Kaposi Sarcoma and Other Skin Diseases in Resource-Limited Settings.Dermatologic clinics · 2021Review
- Pharmacologic management ofExpert review of clinical pharmacology · 2020Review
- Global Epidemiology of Buruli Ulcer, 2010-2017, and Analysis of 2014 WHO Programmatic Targets.Emerging infectious diseases · 2019Article
- Recombinant Antibodies against Mycolactone.Toxins · 2019Article
- Article
- Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay.PLoS neglected tropical diseases · 2019Article
- Integrated Management of Skin NTDs-Lessons Learned from Existing Practice and Field Research.Tropical medicine and infectious disease · 2018Review
- Buruli Ulcer: Review of a Neglected Skin Mycobacterial Disease.Journal of clinical microbiology · 2018Review
- Buruli Ulcer, a Prototype for Ecosystem-Related Infection, Caused by Mycobacterium ulcerans.Clinical microbiology reviews · 2018Review
- Buruli Ulcer: a Review of the Current Knowledge.Current tropical medicine reports · 2018Review
- Analysis of Mycobacterium ulcerans-specific T-cell cytokines for diagnosis of Buruli ulcer disease and as potential indicator for disease progression.PLoS neglected tropical diseases · 2017Article
- Integrated Control and Management of Neglected Tropical Skin Diseases.PLoS neglected tropical diseases · 2017Article
- The chemistry and biology of mycolactones.Beilstein journal of organic chemistry · 2017Review
Corrections and comments
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Authors and funding
13 authors at 7 institutions in 6 countries.
Funding
Abstract
introductionMycobacterium ulcerans infection, known as Buruli ulcer, is a disease of the skin and subcutaneous tissues which is an important but neglected tropical disease with its major impact in rural parts of West and Central Africa where facilities for diagnosis and management are poorly developed. We evaluated fluorescent thin layer chromatography (f-TLC) for detection of mycolactone in the laboratory using samples from patients with Buruli ulcer and patients with similar lesions that gave a negative result on PCR for the IS2404 repeat sequence of M. ulcerans. METHODOLOGY/PRINCIPAL
findingsMycolactone and DNA extracts from fine needle aspiration (FNA), swabs and biopsy specimen were used to determine the sensitivity and specificity of f-TLC when compared with PCR for the IS2404. For 71 IS2404 PCR positive and 28 PCR negative samples the sensitivity was 73.2% and specificity of 85.7% for f-TLC. The sensitivity was similar for swabs (73%), FNAs (75%) and biopsies (70%).
conclusionsWe have shown that mycolactone can be detected from M. ulcerans infected skin tissue by f-TLC technique. The technique is simple, easy to perform and read with minimal costs. In this study it was undertaken by a member of the group from each endemic country. It is a potentially implementable tool at the district level after evaluation in larger field studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.