Evidence map›Paper›PMID 26582930›Full record

ArticleNeuro-oncology2016

Homeobox C9 suppresses Beclin1-mediated autophagy in glioblastoma by directly inhibiting the transcription of death-associated protein kinase 1.

Fan Xuan, Mengying Huang, Wen Liu, Hanfei Ding, Liqun Yang, Hongjuan Cui

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 41 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Fan XuanState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Mengying HuangState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Wen LiuState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Hanfei DingState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Liqun YangState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Hongjuan CuiState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China (F.X., M.H., W.L., L.Y., H.C.); Department of Pathology, Medical College of Georgia, Georgia Regents University, Augusta, Georgia (H.D.).
Augusta University · USSouthwest University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe transcription factor homeobox C9 (HOXC9) plays a crucial role in developmental regulatory systems, where it determines the specific positional identities of cells along the anteroposterior axis. The expression of HOXC9 has been found to be dysregulated in some cancers such as lung cancer, breast cancer, and neuroblastoma. Here, we report for the first time that HOXC9 is a novel autophagy regulator and reveal its oncogenic role in cell survival and its usefulness as a prognostic marker in glioblastoma patients.

methodsKaplan-Meier analysis was performed to evaluate the possible prognostic value of HOXC9 in glioblastoma. Growth curve assays, subcutaneous, and orthotopic implantations were used to analyze cell viability and tumor formation, respectively. Luciferase and chromatin immunoprecipitation assays were employed to explore the mechanisms involved in the association between HOXC9 and its downstream effector, death-associated protein kinase 1 (DAPK1).

resultsHigh expression of HOXC9 was found to be an indicator of a poor prognosis in glioblastoma. HOXC9 knockdown resulted in a significant reduction of cell viability, migration, invasion, and tumorigenicity and a marked increase in autophagy. During the autophagy process, HOXC9 inhibited DAPK1 transcription by directly binding to its promoter. The downregulation of HOXC9 releases its transcriptional inhibition of DAPK1, resulting in the activation of the DAPK1-Beclin1 pathway, which induces autophagy in glioblastoma cells.

conclusionsCollectively, our data indicate that HOXC9 is an oncogene in glioblastoma. We have revealed its role in the control of autophagy, and we suggest that HOXC9 is a novel and promising therapeutic target.

Indexed as

ApoptosisAutophagyBeclin-1Cell Line, TumorCell ProliferationCell SurvivalDeath-Associated Protein KinasesGlioblastomaHomeodomain ProteinsHumansNeuroblastomaPromoter Regions, GeneticSignal TransductionBeclin-1DAPK1 protein, humanDeath-Associated Protein KinasesHMBOX1 protein, humanHomeodomain ProteinsHoxc9 protein, humanautophagyDAPK1glioblastomaHOXC9tumorigenesis

Identifiers

PMID26582930
PMCPMC4864258
OpenAlexW2286002846

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.