ArticleBMC genomics2015
Complete depletion of primordial germ cells in an All-female fish leads to Sex-biased gene expression alteration and sterile All-male occurrence.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- amh alleles on XY sex chromosomes regulate male differentiation in amphidiploid Carassius auratus.Science China. Life sciences · 2026Article
- Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).Scientific reports · 2025Article
- Early fasting does not impact gonadal size nor vasa gene expression in the European seabass Dicentrarchus labrax.Fish physiology and biochemistry · 2024Article
- Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation.Fish physiology and biochemistry · 2023Article
- Spinyhead Croaker Germ Cells GeneLife (Basel, Switzerland) · 2022Article
- Complete Depletion of Primordial Germ Cells Results in Masculinization of Monopterus albus, a Protogynous Hermaphroditic Fish.Marine biotechnology (New York, N.Y.) · 2022Article
- Epigenetic Regulation of Phenotypic Sexual Plasticity Inducing Skewed Sex Ratio in Zebrafish.Frontiers in cell and developmental biology · 2022Article
- Functional Divergence of Multiple Duplicated Foxl2 Homeologs and Alleles in a Recurrent Polyploid Fish.Molecular biology and evolution · 2021Article
- The possible involvement of oxidative stress in the oocyte ageing process in goldfish Carassius auratus (Linnaeus, 1758).Scientific reports · 2019Article
- Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation.Nature communications · 2019Article
- Genetic mechanism underlying sexual plasticity and its association with colour patterning in zebrafish (Danio rerio).BMC genomics · 2019Article
- Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline.Marine biotechnology (New York, N.Y.) · 2019Article
- Phenotypic plasticity induced using high ambient temperature during embryogenesis in domesticated zebrafish, Danio rerio.Reproduction in domestic animals = Zuchthygiene · 2019Article
- Alteration of mRNA abundance, oxidation products and antioxidant enzyme activities during oocyte ageing in common carp Cyprinus carpio.PloS one · 2019Article
- The gonadal transcriptome of the unisexual Amazon molly Poecilia formosa in comparison to its sexual ancestors, Poecilia mexicana and Poecilia latipinna.BMC genomics · 2018Article
- Distinct and Cooperative Roles ofGenetics · 2017Article
- Sex steroid production associated with puberty is absent in germ cell-free salmon.Scientific reports · 2017Article
- MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish.Scientific reports · 2017Article
- Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes.BMC genomics · 2017Article
- Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides.International journal of molecular sciences · 2017Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGynogenesis is one of unisexual reproduction modes in vertebrates, and produces all-female individuals with identical genetic background. In sexual reproduction vertebrates, the roles of primordial germ cells on sexual dimorphism and gonadal differentiation have been largely studied, and two distinct functional models have been proposed. However, the role of primordial germ cells remains unknown in unisexual animals, and it is also unclear whether the functional models in sexual reproduction animals are common in unisexual animals.
resultsTo solve these puzzles, we attempt to utilize the gynogenetic superiority of polyploid Carassius gibelio to create a complete germ cell-depleted gonad model by a similar morpholino-mediated knockdown approach used in other examined sexual reproduction fishes. Through the germ cell-depleted gonad model, we have performed comprehensive and comparative transcriptome analysis, and revealed a complete alteration of sex-biased gene expression. Moreover, the expression alteration leads to up-regulation of testis-biased genes and down-regulation of ovary-biased genes, and results in the occurrence of sterile all-males with testis-like gonads and secondary sex characteristics in the germ cell-depleted gynogenetic Carassius gibelio.
conclusionsOur current results have demonstrated that unisexual gynogenetic embryos remain keeping male sex determination information in the genome, and the complete depletion of primordial germ cells in the all-female fish leads to sex-biased gene expression alteration and sterile all-male occurrence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.