ArticleNature communications2015
Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 65 citations in OpenAlex.
- Spinach-based RNA mimicking GFP in plant cells.Functional & integrative genomics · 2022Article
- Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.Molecular & cellular proteomics : MCP · 2022Review
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Article
- Applications of phage-derived RNA-based technologies in synthetic biology.Synthetic and systems biotechnology · 2020Review
- Following the messenger: Recent innovations in live cell single molecule fluorescence imaging.Wiley interdisciplinary reviews. RNA · 2020Review
- A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells.Nature chemical biology · 2020Article
- From fluorescent proteins to fluorogenic RNAs: Tools for imaging cellular macromolecules.Protein science : a publication of the Protein Society · 2019Review
- RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.Angewandte Chemie (International ed. in English) · 2019Review
- Fluorogenic RNA Aptamers: A Nano-platform for Fabrication of Simple and Combinatorial Logic Gates.Nanomaterials (Basel, Switzerland) · 2018Article
- Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines.Nature communications · 2018Article
- Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells.Nature communications · 2018Article
- Nab3's localization to a nuclear granule in response to nutrient deprivation is determined by its essential prion-like domain.PloS one · 2018Article
- An improved MS2 system for accurate reporting of the mRNA life cycle.Nature methods · 2018Article
- Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.International journal of molecular sciences · 2017Review
- Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI.RNA (New York, N.Y.) · 2017Article
- FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs.Nucleic acids research · 2017Article
- Nuclear pore complexes and regulation of gene expression.Current opinion in cell biology · 2017Review
- Modifications of the chromophore of Spinach aptamer based on QM:MM calculations.Journal of molecular modeling · 2017Article
- Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Although many factors required for the formation of export-competent mRNPs have been described, an integrative view of the spatiotemporal coordinated cascade leading mRNPs from their site of transcription to their site of nuclear exit, at a single cell level, is still partially missing due to technological limitations. Here we report that the RNA Spinach aptamer is a powerful tool for mRNA imaging in live S. cerevisiae with high spatial-temporal resolution and no perturbation of the mRNA biogenesis properties. Dedicated image processing workflows are developed to allow detection of very low abundance of transcripts, accurate quantitative dynamic studies, as well as to provide a localization precision close to 100 nm at consistent time scales. Combining these approaches has provided a state-of-the-art analysis of the osmotic shock response in live yeast by localizing induced transcription factors, target gene loci and corresponding transcripts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.