ArticleScientific reports2015
The Specification and Maturation of Nociceptive Neurons from Human Embryonic Stem Cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Molecular and cellular neurocardiology in heart disease.The Journal of physiology · 2025Review
- Development of a functional human induced pluripotent stem cell-derived nociceptor MEA system as a pain model for analgesic drug testing.Frontiers in cell and developmental biology · 2023Article
- Isolation of human pluripotent stem cell-derived sensory neuron subtypes by immunopanning.Frontiers in cell and developmental biology · 2023Article
- In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system.Scientific reports · 2022Article
- Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization.Cells · 2022Article
- Towards bridging the translational gap by improved modeling of human nociception in health and disease.Pflugers Archiv : European journal of physiology · 2022Review
- Generation of Human Nociceptor-Enriched Sensory Neurons for the Study of Pain-Related Dysfunctions.Stem cells translational medicine · 2022Article
- Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro.International journal of molecular sciences · 2022Article
- Engineering human skin model innervated with itch sensory neuron-like cells differentiated from induced pluripotent stem cells.Bioengineering & translational medicine · 2022Article
- NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Derivation of Peripheral Nociceptive, Mechanoreceptive, and Proprioceptive Sensory Neurons from the same Culture of Human Pluripotent Stem Cells.Stem cell reports · 2021Article
- In Vitro Differentiation of Human Skin-Derived Cells into Functional Sensory Neurons-Like.Cells · 2020Article
- Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons.Frontiers in cellular neuroscience · 2020Article
- Induced pluripotent stem cells for neural drug discovery.Drug discovery today · 2019Review
- A novel method to reconstruct epithelial tissue using high-purity keratinocyte lineage cells induced from human embryonic stem cells.Cell cycle (Georgetown, Tex.) · 2019Article
- Development and validation of an in vitro model system to study peripheral sensory neuron development and injury.Scientific reports · 2018Article
- Representing Diversity in the Dish: Using Patient-DerivedFrontiers in neuroscience · 2018Review
- Human peptidergic nociceptive sensory neurons generated from human epidermal neural crest stem cells (hEPI-NCSC).PloS one · 2018Article
- Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Parallel Human Differentiation Into Sensory Neural Subtypes.Cellular reprogramming · 2017Article
- Cellular models of pain: New technologies and their potential to progress preclinical research.Neurobiology of pain (Cambridge, Mass.)Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Nociceptive neurons play an essential role in pain sensation by transmitting painful stimuli to the central nervous system. However, investigations of nociceptive neuron biology have been hampered by the lack of accessibility of human nociceptive neurons. Here, we describe a system for efficiently guiding human embryonic stem cells into nociceptive neurons by first inducing these cells to the neural lineage. Subsequent addition of retinoic acid and BMP4 at specific time points and concentrations yielded a high population of neural crest progenitor cells (AP2α(+), P75(+)), which further differentiated into nociceptive neurons (TRKA(+), Nav1.7(+), P2X3(+)). The overexpression of Neurogenin 1 (Neurog1) promoted the neurons to express genes related to sensory neurons (Peripherin, TrkA) and to further mature into TRPV1(+) nociceptive neurons. Importantly, the overexpression of Neurog1 increased the response of these neurons to capsaicin stimulation, a hallmark of mature functional nociceptive neurons. Taken together, this study reveals the important role that Neurog1 plays in generating functional human nociceptive neurons.
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