ArticleThe Journal of steroid biochemistry and molecular biology2016
Development of a novel cell based androgen screening model.
Article in The Journal of steroid biochemistry and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- 24-Hour Profiles of 11-Oxygenated CFrontiers in endocrinology · 2021Trial
- An in Vitro triple screen model for human mineralocorticoid receptor activity.The Journal of steroid biochemistry and molecular biology · 2024Article
- Evaluation of 3β-hydroxysteroid dehydrogenase activity using progesterone and androgen receptors-mediated transactivation.Frontiers in endocrinology · 2024Article
- C11-hydroxy and C11-oxo CInternational journal of molecular sciences · 2023Article
- Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort.Endocrine connections · 2023Article
- The role of 11-oxygenated androgens in prostate cancer.Endocrine oncology (Bristol, England) · 2023Review
- Article
- Differences of adrenal-derived androgens in 5α-reductase deficiency versus androgen insensitivity syndrome.Clinical and translational science · 2022Article
- 11-Oxyandrogens from the viewpoint of pediatric endocrinology.Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology · 2022Review
- Normal and Premature Adrenarche.Endocrine reviews · 2021Review
- 11βHSD2 Efficacy in Preventing Transcriptional Activation of the Mineralocorticoid Receptor by Corticosterone.Journal of the Endocrine Society · 2021Article
- Circadian rhythms of 11-oxygenated C19 steroids and ∆5-steroid sulfates in healthy men.European journal of endocrinology · 2021Article
- Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells.BioTechniques · 2021Article
- Osteoblasts Generate Testosterone From DHEA and Activate Androgen Signaling in Prostate Cancer Cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- 11-Oxygenated Androgens Useful in the Setting of Discrepant Conventional Biomarkers in 21-Hydroxylase Deficiency.Journal of the Endocrine Society · 2021Article
- A Genetically Encoded Bioluminescence Intracellular Nanosensor for Androgen Receptor Activation Monitoring in 3D Cell Models.Sensors (Basel, Switzerland) · 2021Article
- Disorders of Sex Development of Adrenal Origin.Frontiers in endocrinology · 2021Review
- Sex Differences in 11-Oxygenated Androgen Patterns Across Adulthood.The Journal of clinical endocrinology and metabolism · 2020Article
- Update on adrenarche.Current opinion in pediatrics · 2020Review
- 11-Oxygenated androgens in health and disease.Nature reviews. Endocrinology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The androgen receptor (AR) mediates the majority of androgen effects on target cells. The DNA cis-regulatory elements that respond to AR share sequence similarity with cis-regulatory elements for glucocorticoid, mineralocorticoid and progesterone receptors (GR, MR and PR, respectively). As a result, many of the current AR screening models are complicated by inaccurate activation of reporters by one of these receptor pathways. Identification of more selective androgen testing systems would be beneficial for clinical, pharmacological and toxicologic screening of AR activators. The present study describes the development of a selective androgen-responsive reporter cell line that expresses AR but does not express GR, MR and PR. CV1 cells were stably transduced to express human AR and an androgen-responsive gaussia luciferase gene. Clonal populations of AR expressing cells were isolated. Quantitative RT-PCR (qPCR) and western analysis confirmed stable integration of AR in the most responsive clonal line which was named 'CV1-ARluc'. Stimulation of CV1AR-luc with androgenic ligands (testosterone and 5α-dihydrotestosterone) for 18h caused an increase in luciferase activity in a dose-dependent manner. Other steroid hormones including aldosterone, cortisol, and progesterone did not stimulate luciferase response. The CV1-ARluc also increased luciferase activity when treated with human serum extracts. In conclusion, the CV1-ARluc cells provide a novel model system for screening of new AR agonists and antagonists and can determine the androgenic activity of human serum samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.