ArticlePloS one2015
Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 32 citations in OpenAlex.
- A hickory diacylglycerol acyltransferase gene coordinates fatty acid metabolism and aluminum tolerance in plants.The New phytologist · 2026Article
- Identification of a promoter region specifically active in the maturing endosperm of Arabidopsis seeds and its use for targeted modification of fatty acid metabolism.The Plant journal : for cell and molecular biology · 2025Article
- Article
- A yeast-based tool for screening mammalian diacylglycerol acyltransferase inhibitors.MicrobiologyOpen · 2022Article
- Chromosome-level genome assembly and resequencing of camphor tree (Horticulture research · 2022Article
- Acyl-CoA-dependent and acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols.Frontiers in plant science · 2022Article
- Molecular Control of Oil Metabolism in the Endosperm of Seeds.International journal of molecular sciences · 2021Review
- Functional characterization of two type-1 diacylglycerol acyltransferase (DGAT1) genes from rice (Oryza sativa) embryo restoring the triacylglycerol accumulation in yeast.Plant molecular biology · 2021Article
- Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm.Plant & cell physiology · 2019Article
- Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis.Scientific reports · 2018Article
- Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering.Microbial cell factories · 2018Article
- Seeds as oil factories.Plant reproduction · 2018Review
- A Specialized Diacylglycerol Acyltransferase Contributes to the Extreme Medium-Chain Fatty Acid Content ofPlant physiology · 2017Article
- Characterization and Functional Analysis of a Type 2 Diacylglycerol Acyltransferase (Frontiers in plant science · 2017Article
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4 authors at 2 institutions in 1 country.
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Abstract
Diacylglycerol acyltransferases (DGAT) are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm) seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0). A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1) is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting that it is a determinant of medium-chain triacylglycerol storage. To test this hypothesis, we thoroughly characterized EgDGAT1-1 activity through functional complementation of a Yarrowia lipolytica mutant strain devoid of neutral lipids. EgDGAT1-1 expression is sufficient to restore triacylglycerol accumulation in neosynthesized lipid droplets. A comparative functional study with Arabidopsis thaliana DGAT1 highlighted contrasting substrate specificities when the recombinant yeast was cultured in lauric acid supplemented medium. The EgDGAT1-1 expressing strain preferentially accumulated medium-chain triacylglycerols whereas AtDGAT1 expression induced long-chain triacylglycerol storage in Y. lipolytica. EgDGAT1-1 localized to the endoplasmic reticulum where TAG biosynthesis takes place. Reestablishing neutral lipid accumulation in the Y. lipolytica mutant strain did not induce major reorganization of the yeast microsomal proteome. Overall, our findings demonstrate that EgDGAT1-1 is an endoplasmic reticulum DGAT with preference for medium-chain fatty acid substrates, in line with its physiological role in palm kernel. The characterized EgDGAT1-1 could be used to promote medium-chain triacylglycerol accumulation in microbial-produced oil for industrial chemicals and cosmetics.
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