ArticlePloS one2015
Simple, Low-Cost Detection of Candida parapsilosis Complex Isolates and Molecular Fingerprinting of Candida orthopsilosis Strains in Kuwait by ITS Region Sequencing and Amplified Fragment Length Polymorphism Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 55 citations in OpenAlex.
- Candidemia in a General Hospital in Kuwait: Epidemiology, Species Distribution, Risk Factors, and Antifungal Susceptibility Patterns over a 10-Year Period (2015-2024).Journal of fungi (Basel, Switzerland) · 2025Article
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- Trends in Prevalence of Yeast Species Associated With Urogenital Infection in Nsukka, Nigeria: An Overview of TrueInternational journal of microbiology · 2025Article
- Evaluation of Etest and MICRONAUT-AM Assay for Antifungal Susceptibility Testing ofAntibiotics (Basel, Switzerland) · 2024Article
- The first established microsatellite markers to distinguishJournal of clinical microbiology · 2023Article
- Molecular Characterization and Sterol Profiles Identify Nonsynonymous Mutations inMicrobiology spectrum · 2023Article
- Species distribution and susceptibility profiles of oral candidiasis in hematological malignancy and solid tumor patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Molecular fingerprinting by multi-locus sequence typing identifies microevolution and nosocomial transmission ofFrontiers in public health · 2023Article
- FatalJournal of fungi (Basel, Switzerland) · 2022Article
- Fatal Breakthrough Candidemia in an Immunocompromised Patient in Kuwait Due toJournal of fungi (Basel, Switzerland) · 2022Article
- Epidemiology of Candidemia in Kuwait: A Nationwide, Population-Based Study.Journal of fungi (Basel, Switzerland) · 2021Article
- Multiplex PCR method for simultaneous detection of five pathogenic bacteria closely related to foodborne diseases.3 Biotech · 2021Article
- Review
- First Isolation of Candida nivariensis, an Emerging Fungal Pathogen, in Kuwait.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2021Article
- Molecular Epidemiology ofJournal of fungi (Basel, Switzerland) · 2020Article
- Concurrent bloodstream infection withMedical mycology case reports · 2020Article
- Antifungal drug susceptibility, molecular basis of resistance to echinocandins and molecular epidemiology of fluconazole resistance among clinical Candida glabrata isolates in Kuwait.Scientific reports · 2020Article
- Candida kefyr in Kuwait: Prevalence, antifungal drug susceptibility and genotypic heterogeneity.PloS one · 2020Article
- Identification of Cryptic Species of FourJournal of fungi (Basel, Switzerland) · 2019Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida parapsilosis has now emerged as the second or third most important cause of healthcare-associated Candida infections. Molecular studies have shown that phenotypically identified C. parapsilosis isolates represent a complex of three species, namely, C. parapsilosis, C. orthopsilosis and C. metapsilosis. Lodderomyces elongisporus is another species phenotypically closely related to the C. parapsilosis-complex. The aim of this study was to develop a simple, low cost multiplex (m) PCR assay for species-specific identification of C. parapsilosis complex isolates and to study genetic relatedness of C. orthopsilosis isolates in Kuwait. Species-specific amplicons from C. parapsilosis (171 bp), C. orthopsilosis (109 bp), C. metapsilosis (217 bp) and L. elongisporus (258 bp) were obtained in mPCR. Clinical isolates identified as C. parapsilosis (n = 380) by Vitek2 in Kuwait and an international collection of 27 C. parapsilosis complex and L. elongisporus isolates previously characterized by rDNA sequencing were analyzed to evaluate mPCR. Species-specific PCR and DNA sequencing of internal transcribed spacer (ITS) region of rDNA were performed to validate the results of mPCR. Fingerprinting of 19 clinical C. orthopsilosis isolates (including 4 isolates from a previous study) was performed by amplified fragment length polymorphism (AFLP) analysis. Phenotypically identified C. parapsilosis isolates (n = 380) were identified as C. parapsilosis sensu stricto (n = 361), C. orthopsilosis (n = 15), C. metapsilosis (n = 1) and L. elongisporus (n = 3) by mPCR. The mPCR also accurately detected all epidemiologically unrelated C. parapsilosis complex and L. elongisporus isolates. The 19 C. orthopsilosis isolates obtained from 16 patients were divided into 3 haplotypes based on ITS region sequence data. Seven distinct genotypes were identified among the 19 C. orthopsilosis isolates by AFLP including a dominant genotype (AFLP1) comprising 11 isolates recovered from 10 patients. A rapid, low-cost mPCR assay for detection and differentiation of C. parapsilosis, C. orthopsilosis, C. metapsilosis and L. elongisporus has been developed.
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