ArticlePloS one2015
Comprehensive Analysis of a Yeast Lipase Family in the Yarrowia Clade.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Taxonomic Identification of Two Novel Genera and Four Novel Species of Lipolytic Floral-Associated Yeasts.Journal of fungi (Basel, Switzerland) · 2026Article
- Identification and functional analysis of growth rate associated long non-coding RNAs inComputational and structural biotechnology journal · 2025Article
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- Article
- An Appraisal on Prominent Industrial and Biotechnological Applications of Bacterial Lipases.Molecular biotechnology · 2023Review
- Comparative Analysis of the Alkaline Proteolytic Enzymes ofInternational journal of molecular sciences · 2023Article
- Insights into the Genomic and Phenotypic Landscape of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2023Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Improved production of biocatalysts by Yarrowia lipolytica using natural sources of the biopolyesters cutin and suberin, and their application in hydrolysis of poly (ethylene terephthalate) (PET).Bioprocess and biosystems engineering · 2021Article
- High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast.Microbial cell factories · 2021Article
- The Occurrence of Triple Catalytic Characteristics of Yeast Lipases and Their Application Prospects in Biodiesel Production from Non-Edible Jatropha curcas Oil in a Solvent-Free System.Current microbiology · 2021Article
- Phylogenomic and biochemical analysis reassesses temperate marine yeast Yarrowia lipolytica NCIM 3590 to be Yarrowia bubula.Scientific reports · 2021Article
- Identification of telomerase RNAs in species of the Yarrowia clade provides insights into the co-evolution of telomerase, telomeric repeats and telomere-binding proteins.Scientific reports · 2019Article
- New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipases are currently the subject of intensive studies due to their large range of industrial applications. The Lip2p lipase from the yeast Yarrowia lipolytica (YlLIP2) was recently shown to be a good candidate for different biotechnological applications. Using a combination of comparative genomics approaches based on sequence similarity, synteny conservation, and phylogeny, we constructed the evolutionary scenario of the lipase family for six species of the Yarrowia clade. RNA-seq based transcriptome analysis revealed the primary role of LIP2 homologues in the assimilation of different substrates. Once identified, these YlLIP2 homologues were expressed in Y. lipolytica. The lipase Lip2a from Candida phangngensis was shown to naturally present better activity and enantioselectivity than YlLip2. Enantioselectivity was further improved by site-directed mutagenesis targeted to the substrate binding site. The mono-substituted variant V232S displayed enantioselectivity greater than 200 and a 2.5 fold increase in velocity. A double-substituted variant 97A-V232F presented reversed enantioselectivity, with a total preference for the R-enantiomer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.