ArticleRNA biology2016
RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes.
Article in RNA biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Translation Inhibiting Antibiotics Induce an asRNA Regulating Purine Metabolism inbioRxiv : the preprint server for biology · 2026Article
- RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteria.Nature communications · 2025Article
- Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability.Nucleic acids research · 2024Article
- Dynamic Protein Phosphorylation inMicroorganisms · 2024Article
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- Insights into 6S RNA in lactic acid bacteria (LAB).BMC genomic data · 2021Article
- An Inventory of CiaR-Dependent Small Regulatory RNAs inFrontiers in microbiology · 2021Review
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- Article
- Large-scale and significant expression from pseudogenes inMicrobial genomics · 2020Article
- Conserved and specific features of Streptococcus pyogenes and Streptococcus agalactiae transcriptional landscapes.BMC genomics · 2019Article
- Article
- In vivo 3'-to-5' exoribonuclease targetomes ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Functional annotation and distribution overview of RNA families in 27 Streptococcus agalactiae genomes.BMC genomics · 2018Article
- A Glycine Riboswitch inFrontiers in microbiology · 2018Article
- RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B.RNA biology · 2018Article
- Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes.Nucleic acids research · 2017Article
- RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts inFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pyogenes is a human pathogen responsible for a wide spectrum of diseases ranging from mild to life-threatening infections. During the infectious process, the temporal and spatial expression of pathogenicity factors is tightly controlled by a complex network of protein and RNA regulators acting in response to various environmental signals. Here, we focus on the class of small RNA regulators (sRNAs) and present the first complete analysis of sRNA sequencing data in S. pyogenes. In the SF370 clinical isolate (M1 serotype), we identified 197 and 428 putative regulatory RNAs by visual inspection and bioinformatics screening of the sequencing data, respectively. Only 35 from the 197 candidates identified by visual screening were assigned a predicted function (T-boxes, ribosomal protein leaders, characterized riboswitches or sRNAs), indicating how little is known about sRNA regulation in S. pyogenes. By comparing our list of predicted sRNAs with previous S. pyogenes sRNA screens using bioinformatics or microarrays, 92 novel sRNAs were revealed, including antisense RNAs that are for the first time shown to be expressed in this pathogen. We experimentally validated the expression of 30 novel sRNAs and antisense RNAs. We show that the expression profile of 9 sRNAs including 2 predicted regulatory elements is affected by the endoribonucleases RNase III and/or RNase Y, highlighting the critical role of these enzymes in sRNA regulation.
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