Evidence map›Paper›PMID 26580231›Full record

ArticleACS nano2016

Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.

Nalan Liv, Daan S B van Oosten Slingeland, Jean-Pierre Baudoin, Pieter Kruit, David W Piston, Jacob P Hoogenboom

Abstract read
In one paragraph

Article in ACS nano, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. The 2018 correlative microscopy techniques roadmap.Journal of physics D: Applied physics · 2018
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nalan LivDepartment of Imaging Physics, Delft University of Technology , Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Daan S B van Oosten SlingelandDepartment of Imaging Physics, Delft University of Technology , Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Jean-Pierre BaudoinDepartment of Molecular Physiology and Biophysics, Vanderbilt University , 747 Light Hall, Nashville, Tennessee 37232-0615, United States.
Pieter KruitDepartment of Imaging Physics, Delft University of Technology , Lorentzweg 1, 2628 CJ Delft, The Netherlands.
David W PistonDepartment of Molecular Physiology and Biophysics, Vanderbilt University , 747 Light Hall, Nashville, Tennessee 37232-0615, United States.
Jacob P HoogenboomDepartment of Imaging Physics, Delft University of Technology , Lorentzweg 1, 2628 CJ Delft, The Netherlands.

Funding

Three dimensional aberration corrected scanning transmission electron microscopyR01GM081801 · NIGMS · VANDERBILT UNIVERSITY · PI PISTON, DAVID W · 2008 to 2011
$1.4M
Pancreatic Islets Dynamics Regulating Glucagon SecretionR01DK098659 · NIDDK · WASHINGTON UNIVERSITY · PI PISTON, DAVID W · 2013 to 2016
$1.3M
Parallel Computer with High Memory NodesS10OD016216 · OD · VANDERBILT UNIVERSITY · PI PISTON, DAVID W · 2013 to 2013
$598k
Multi-Function Laser Scanning MicroscopeS10RR025649 · NCRR · VANDERBILT UNIVERSITY · PI PISTON, DAVID W · 2009 to 2009
$499k
Multiphoton microscope upgradeS10OD010681 · OD · VANDERBILT UNIVERSITY · PI PISTON, DAVID W · 2012 to 2012
$436k
NCRR NIH HHS S10 RR025649NIDDK NIH HHS R01 DK098659NIGMS NIH HHS R01 GM081801NIH HHS S10 OD010681NIH HHS S10 OD016216
6 · The paper itself

Abstract

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.

Indexed as

AnimalsBioreactorsCell LineCells, ImmobilizedChlorocebus aethiopsEndocytosisFibroblastsMicroscopy, Electron, ScanningOptical ImagingQuantum Dotsepidermal growth factorfluorescence microscopylive cell imagingquantum dotsscanning electron microscopy

Identifiers

PMID26580231
PMCPMC4729641

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.