Evidence map›Paper›PMID 26580119›Full record

ArticleCellular reprogramming2015

Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells.

Huijun Shi, Qiang Fu, Guozhong Li, Yan Ren, Shengwei Hu, Wei Ni, Fei Guo, Mengting Shi, Luping Meng, Hui Zhang and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular reprogramming, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Induced pluripotent stem cells from farm animals.Journal of animal science · 2020
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Huijun Shi1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Qiang Fu1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Guozhong Li1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Yan Ren3 College of Medicine, Shihezi University , Shihezi 832003, Xinjiang, China .
Shengwei Hu4 College of Life Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Wei Ni4 College of Life Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Fei Guo3 College of Medicine, Shihezi University , Shihezi 832003, Xinjiang, China .
Mengting Shi4 College of Life Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Luping Meng1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Hui Zhang1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Jun Qiao1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Zhiru Guo1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Chuangfu Chen1 College of Animal Science and Technology, Shihezi University , Shihezi 832003, Xinjiang, China .
Shihezi University · CNXinjiang Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Since the first report of induced pluripotent stem cells (iPSCs) by Takahashi and Yamanaka, numerous attempts have been made to derive iPSCs from other species via the ectopic expression of defined factors. Sheep iPSCs (siPSCs) have significant potential for biotechnology and agriculture. Although several groups have described siPSCs, the reprogramming efficiency was extremely low. The exogenous transgenes could be not silenced in the iPSCs, which hampered their development and application. Here, we report that p53 knockdown and antisilencing function 1A (ASF1A) overexpression promoted iPSC generation from sheep kidney cells (SKCs). Compared with transduction with eight human defined transcription factors (Oct4, Sox2, Klf4, c-Myc, Nanog, Lin28, hTERT, and SV40LT), the additional introduction of p53 RNA interference (RNAi) and/or ASF1A in the presence of small-molecule compounds [vitamin C (Vc) and valproic acid (VPA)] greatly improved the efficiency of sheep iPSC generation. The siPSCs exhibited morphological features similar to mouse embryonic stem cells (ESCs) and were positive for alkaline phosphatase and, pluripotent marker genes (Oct4, Nanog, Sox2, Rex1, TRA-1-60, TRA-1-81, and E-cadherin). Furthermore, these cells exhibited a normal karyotype of 54 chromosomes and were able to differentiate into all three germ layers both in vitro and in vivo. Moreover, the exogenous genes were silenced in siPSCs when p53 small hairpin RNA (shRNA) and ASF1A were added. Our results may help to reveal the role of p53 and ASF1A in sheep somatic cell reprogramming and provide an efficient approach to reprogramming sheep somatic cells.

Indexed as

AnimalsCell DifferentiationCellular ReprogrammingChromosomal Proteins, Non-HistoneGene SilencingImmunohistochemistryInduced Pluripotent Stem CellsKaryotypingKidneyKruppel-Like Factor 4LentivirusMiceReal-Time Polymerase Chain ReactionRNA InterferenceRNA, Small InterferingSheepChromosomal Proteins, Non-HistoneKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4RNA, Small InterferingTranscription FactorsTumor Suppressor Protein p53

Identifiers

PMID26580119
PMCPMC4677545
OpenAlexW2172614614

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.