Evidence map›Paper›PMID 26579970›Full record

ArticleCellular reprogramming2015

Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos.

Fuliang Du, Chien-Hong Chen, Yi Li, Yeshu Hu, Li-You An, Lan Yang, Jifeng Zhang, Y Eugene Chen, Jie Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular reprogramming, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Generation of Rabbit Chimeras by Eight-Cell Stage Embryo Injection.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  4. Genome engineering technologies in rabbits.Journal of biomedical research · 2020
    Article
  5. Article
  6. Deriving rabbit embryonic stem cells by small molecule inhibitors.American journal of translational research · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Fuliang Du1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
Chien-Hong Chen2 Renova Life, Inc. , College Park, Maryland 20742.
Yi Li1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
Yeshu Hu1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
Li-You An1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
Lan Yang3 Lannuo Biotechnologies Wuxi, Inc. , Wuxi, Jiangsu 214174, China .
Jifeng Zhang4 Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center , Ann Arbor, MI, 48109.
Y Eugene Chen4 Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center , Ann Arbor, MI, 48109.
Jie Xu2 Renova Life, Inc. , College Park, Maryland 20742.

Funding

CETP and HDL Function in Cardiovascular DiseasesR01HL117491 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE · 2013 to 2016
$2.4M
Derivation of germline competent rabbit embryonic stem cell linesR44RR023774 · NCRR · RENOVA LIFE, INC. · PI DU, FULIANG · 2010 to 2011
$1.1M
NCRR NIH HHS R44RR023774NHLBI NIH HHS R01 HL117491
6 · The paper itself

Abstract

The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.

Indexed as

AmidesAnimalsBlastocystCell DifferentiationCell LineCryopreservationEmbryonic Stem CellsEmbryo TransferFemaleImmunohistochemistryKaryotypingMicePyridinesRabbitsRegenerationTemperatureAmidesPyridinesY 27632

Identifiers

PMID26579970
PMCPMC4677569

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.