ArticlePloS one2015
A Role for Reactive Oxygen Species Produced by NADPH Oxidases in the Embryo and Aleurone Cells in Barley Seed Germination.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 124 citations in OpenAlex.
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Linking Superoxide Production and Scavenging in Plant Development.Plant, cell & environment · 2026Review
- Interplay of different signaling mechanisms in regulating programmed cell death during plant development and in response to stresses.Protoplasma · 2026Review
- Expression Analysis of ROS-Related Genes During the Germination of Chickpea (Cicer Arietinum L.) Seeds.Methods in molecular biology (Clifton, N.J.) · 2026Article
- 2-Hydroxymelatonin Induces Husk-Imposed Vivipary in the Transgenic Rice Overexpressing Melatonin 2-Hydroxylase.Biomolecules · 2025Article
- The F-box protein CHALK10 mediates SEMIDWARF-1 ubiquitination and degradation to negatively regulate grain chalkiness in rice.Plant communications · 2025Article
- Regulation of seed germination: ROS, epigenetic, and hormonal aspects.Journal of advanced research · 2025Review
- Red light induced seed germination and seedling growth by modulating antioxidant defense system, Rubisco, and NADPH oxidase activities in Capsicum frutescens.BMC plant biology · 2025Article
- Regulatory Mechanisms of Bud Dormancy: Environmental, Hormonal, and Genetic Perspectives.International journal of molecular sciences · 2025Review
- Plant responses to heat stress and advances in mitigation strategies.Frontiers in plant science · 2025Review
- Proline and ROS: A Unified Mechanism in Plant Development and Stress Response?Plants (Basel, Switzerland) · 2024Review
- Exogenous abscisic acid improves grain filling capacity under heat stress by enhancing antioxidative defense capability in rice.BMC plant biology · 2023Article
- Imbibitional redox and hormonal priming revealed regulation of oxidative window as a key factor for progression of germination of indica rice cultivars.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (International journal of molecular sciences · 2023Article
- Regulation of reactive oxygen species and phytohormones in osmotic stress tolerance during seed germination inFrontiers in plant science · 2023Article
- Seed dormancy loss from dry after-ripening is associated with increasing gibberellin hormone levels inFrontiers in plant science · 2023Article
- Plant Disease Resistance-Related Signaling Pathways: Recent Progress and Future Prospects.International journal of molecular sciences · 2022Review
- Visualization of Glutamate Decarboxylase Activity in Barley Seeds under Salinity Stress Using Mass Microscope.Metabolites · 2022Article
- Biological Functions of Hydrogen Sulfide in Plants.International journal of molecular sciences · 2022Review
- Heat stress during grain filling regulates seed germination through alterations of DNA methylation in barley (Hordeum vulgare L.).Plant molecular biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consistent with the sites of ROS production in the seed after imbibition. To clarify the role of NADPH oxidases in barley seed germination, we examined gibberellic acid (GA) / abscisic acid (ABA) metabolism and signaling in barley seeds treated with diphenylene iodonium chloride (DPI), an NADPH oxidase inhibitor. DPI significantly suppressed germination, and suppressed GA biosynthesis and ABA catabolism in embryos. GA, but not ABA, induced NADPH oxidase activity in aleurone cells. Additionally, DPI suppressed the early induction of α-amylase by GA in aleurone cells. These results suggest that ROS produced by NADPH oxidases promote GA biosynthesis in embryos, that GA induces and activates NADPH oxidases in aleurone cells, and that ROS produced by NADPH oxidases induce α-amylase in aleurone cells. We conclude that the ROS generated by NADPH oxidases regulate barley seed germination through GA / ABA metabolism and signaling in embryo and aleurone cells.
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