ArticleBioMed research international2015
Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Monocyte Activation and Ageing Biomarkers in the Development of Cardiovascular Ischaemic Events or Diabetes in People with HIV.Microorganisms · 2023Article
- Could long-term dialysis vintage and abnormal calcium, phosphorus and iPTH control accelerate aging among the maintenance hemodialysis population?Renal failure · 2023Article
- Validating biomarkers and models for epigenetic inference of alcohol consumption from blood.Clinical epigenetics · 2021Article
- Inter-laboratory adaption of age estimation models by DNA methylation analysis-problems and solutions.International journal of legal medicine · 2020Article
- Article
- Leukocyte telomere length and epigenetic-based mortality risk score: associations with all-cause mortality among older adults.Epigenetics · 2018Article
- DNA methylation changes in response to active smoking exposure are associated with leukocyte telomere length among older adults.European journal of epidemiology · 2016Article
- Plasma Amyloid Beta 1-42 and DNA Methylation Pattern Predict Accelerated Aging in Young Subjects with Down Syndrome.Neuromolecular medicine · 2016Article
- Forensic DNA methylation profiling from evidence material for investigative leads.BMB reports · 2016Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The environmental factors such as aging, smoking, and alcohol consumption have been reported to influence DNA methylation (DNAm). However, the versatility of DNAm measurement by DNAm array systems is low in clinical use. Thus, we developed the MethyLight assay as a simple method to measure DNAm. In the present study, we isolated peripheral blood DNA from 33 healthy volunteers and selected cg25809905, cg02228185, and cg17861230 as aging, cg23576855 as smoking, and cg02583484 as alcohol consumption biomarkers. The predicted age by methylation rates of cg25809905 and cg17861230 significantly correlated with chronological age. In immortalized B-cells, DNAm rates of two sites showed a younger status than the chronological age of donor. On the other hand, the predicted age of the patients with myocardial infarction (MI) was not accelerated. The methylation rate of cg23576855 was able to discriminate the groups based on the smoking status. The DNAm rate of cg02583484 was reduced in subjects with habitual alcohol consumption compared to that of subjects without habitual alcohol consumption. In conclusion, our MethyLight assay system reconfirms that aging, smoking, and alcohol consumption influenced DNAm in peripheral blood in the Japanese. This MethyLight system will facilitate DNAm measurement in epidemiological and clinical studies.
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