Evidence map›Paper›PMID 26579521›Full record

ArticleFrontiers in cell and developmental biology2015

Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells.

Fernando A Fierro, Adam J O'Neal, Julie R Beegle, Myra N Chávez, Thomas R Peavy, Roslyn R Isseroff, José T Egaña

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Fernando A FierroStem Cell Program, Department of Cell Biology and Human Anatomy, University of California, Davis Davis, CA, USA.
Adam J O'NealDepartment of Biological Sciences, California State University Sacramento, CA, USA.
Julie R BeegleStem Cell Program, Department of Cell Biology and Human Anatomy, University of California, Davis Davis, CA, USA.
Myra N ChávezDepartment of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München Munich, Germany ; Facultad de Ciencias, FONDAP Center for Genome Regulation, Universidad de Chile Santiago, Chile.
Thomas R PeavyDepartment of Biological Sciences, California State University Sacramento, CA, USA.
Roslyn R IsseroffDepartment of Dermatology, University of California, Davis Davis, CA, USA.
José T EgañaDepartment of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München Munich, Germany ; Facultad de Ciencias, FONDAP Center for Genome Regulation, Universidad de Chile Santiago, Chile ; Institute for Medical and Biological Engineering, Schools of Engineering, Biological Sciences and Medicine, Pontificia Universidad Católica de Chile Santiago, Chile.
University of California, Davis · USCalifornia State University, Sacramento · USKlinikum rechts der Isar · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many therapies using mesenchymal stem cells (MSC) rely on their ability to produce and release paracrine signals with chemotactic and pro-angiogenic activity. These characteristics, however, are mostly studied under standard in vitro culture conditions. In contrast, various novel cell-based therapies imply pre-seeding MSC into bio-artificial scaffolds. Here we describe human bone marrow-derived MSC seeded in Integra matrices, a common type of scaffold for dermal regeneration (SDR). We show and measured the distribution of MSC within the SDR, where cells clearly establish physical interactions with the scaffold, exhibiting constant metabolic activity for at least 15 days. In the SDR, MSC secrete VEGF and SDF-1α and induce transwell migration of CD34(+) hematopoietic/endothelial progenitor cells, which is inhibited in the presence of a CXCR4/SDF-1α antagonist. MSC in SDR respond to hypoxia by altering levels of angiogenic signals such as Angiogenin, Serpin-1, uPA, and IL-8. Finally, we show that MSC-containing SDR that have been pre-incubated in hypoxia show higher infiltration of endothelial cells after implantation into immune deficient mice. Our data show that MSC are fully functional ex vivo when implanted into SDR. In addition, our results strongly support the notion of hypoxic pre-conditioning MSC-containing SDR, in order to promote angiogenesis in the wounds.

Indexed as

angiogenesishypoxiamesenchymal stem cellsscaffoldswound healing

Identifiers

PMID26579521
PMCPMC4626656
OpenAlexW2233383916

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.