Evidence map›Paper›PMID 26579456›Full record

ArticleActa pharmaceutica Sinica. B2015

Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres.

Manli Wang, Xiaolong Lu, Xianzhen Yin, Yajun Tong, Weiwei Peng, Li Wu, Haiyan Li, Yan Yang, Jingkai Gu, Tiqiao Xiao and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta pharmaceutica Sinica. B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Manli WangSchool of Pharmaceutical Sciences, Anhui University of Chinese Medicine, Hefei 230038, China ; Center for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Xiaolong LuCenter for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China ; School of Mechanical Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Xianzhen YinCenter for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Yajun TongShanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
Weiwei PengShanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
Li WuCenter for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China ; College of Life Sciences, Jilin University, Changchun 130012, China.
Haiyan LiCenter for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Yan YangCollege of Life Sciences, Jilin University, Changchun 130012, China.
Jingkai GuCollege of Life Sciences, Jilin University, Changchun 130012, China.
Tiqiao XiaoShanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
Min ChenShanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
Jiwen ZhangSchool of Pharmaceutical Sciences, Anhui University of Chinese Medicine, Hefei 230038, China ; Center for Pharmaceutical Preparations, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China ; School of Mechanical Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Shanghai Institute of Applied Physics · CNJilin University · CNChinese Academy of Sciences · CNShanghai Institute of Materia Medica · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide (PLGA) microsphere using synchrotron radiation-based Fourier-transform infrared spectromicroscopy (SR-FTIR). The representative infrared wavenumbers specific for protein/peptide (Exenatide) and excipient (PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands. For quantitative analysis of the distribution within microspheres, Matlab software was used to transform the map file into a 3D matrix and the matrix values specific for the drug and excipient were extracted. Comparison of the normalized SR-FTIR maps of PLGA and Exenatide indicated that PLGA was uniformly distributed, while Exenatide was relatively non-uniformly distributed in the microspheres. In conclusion, SR-FTIR is a rapid, nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres.

Indexed as

ExenatideFourier-transform infrared spectromicroscopyMicrospherePLGAProtein distribution

Identifiers

PMID26579456
PMCPMC4629252
OpenAlexW2056673852

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.