ArticleActa pharmaceutica Sinica. B2014
A cell-based high-throughput approach to identify inhibitors of influenza A virus.
Article in Acta pharmaceutica Sinica. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Identification of the Imidazo[1,2-ACS pharmacology & translational science · 2023Article
- Verbalide A~F: new phthalide derivatives from the endophytic fungus Preussia sp. CPCC 400972.The Journal of antibiotics · 2023Article
- Late-stage cascade of oxidation reactions during the biosynthesis of oxalicine B inActa pharmaceutica Sinica. B · 2023Article
- Discovery of Novel Boron-ContainingMolecules (Basel, Switzerland) · 2022Article
- RNA G-quadruplex formed in SARS-CoV-2 used for COVID-19 treatment in animal models.Cell discovery · 2022Article
- IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.Nature microbiology · 2021Article
- New phenol and chromone derivatives from the endolichenic fungusRSC advances · 2021Article
- Corilagin inhibits SARS-CoV-2 replication by targeting viral RNA-dependent RNA polymerase.Acta pharmaceutica Sinica. B · 2021Article
- New butyrolactone derivatives from the endophytic Fungus Talaromyces sp. CPCC 400783 of Reynoutria japonica Houtt.The Journal of antibiotics · 2021Article
- Daldispones A and B, two new cyclopentenones from Daldinia sp. CPCC 400770.The Journal of antibiotics · 2021Article
- Overview of Viral Pneumonia Associated With Influenza Virus, Respiratory Syncytial Virus, and Coronavirus, and Therapeutics Based on Natural Products of Medicinal Plants.Frontiers in pharmacology · 2021Review
- Microbial Transformation ofFrontiers in microbiology · 2021Article
- Benzothiazoles as potential antiviral agents.The Journal of pharmacy and pharmacology · 2020Review
- Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Article
- Broad-Spectrum Antiviral Natural Products from the Marine-DerivedMolecules (Basel, Switzerland) · 2019Article
- Article
- Novel cell-based in vitro screen to identify small-molecule inhibitors against intracellular replication of Cryptococcus neoformans in macrophages.International journal of antimicrobial agents · 2016Article
- Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner.Biochemistry research international · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Influenza is one of the most common infections threatening public health worldwide and is caused by the influenza virus. Rapid emergence of drug resistance has led to an urgent need to develop new anti-influenza inhibitors. In this study we established a 293T cell line that constitutively synthesizes a virus-based negative strand RNA, which expresses Gaussia luciferase upon influenza A virus infection. Using this cell line, an assay was developed and optimized to search for inhibitors of influenza virus replication. Biochemical studies and statistical analyses presented herein demonstrate the sensitivity and reproducibility of the assay in a high-throughput format (Z' factor value>0.8). A pilot screening provides further evidence for validation of the assay. Taken together, this work provides a simple, convenient, and reliable HTS assay to identify compounds with anti-influenza activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.