ArticleFrontiers in plant science2015
Gene-to-metabolite network for biosynthesis of lignans in MeJA-elicited Isatis indigotica hairy root cultures.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- Insights into the functions of MYB29 and MYB28.1 involved in glucosinolate biosynthesis from Isatis indigotica Fort.Functional & integrative genomics · 2026Article
- GABA-mediated modulation of drought stress tolerance and seed morphology during flax (Linum usitatissimum L.) germination via image analysis.Scientific reports · 2025Article
- De novo biosynthesis of plant lignans by synthetic yeast consortia.Nature chemical biology · 2025Article
- Hairy Roots as Producers of Coumarins, Lignans, and Xanthones.Molecules (Basel, Switzerland) · 2025Review
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- Unveiling the Molecular Mechanisms of Browning inInternational journal of molecular sciences · 2024Article
- Genome sequencing and functional analysis of a multipurpose medicinal herb Tinospora cordifolia (Giloy).Scientific reports · 2024Article
- The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense inActa pharmaceutica Sinica. B · 2024Article
- Biotechnological Approaches to Producing Natural Antioxidants: Anti-Ageing and Skin Longevity Prospects.International journal of molecular sciences · 2023Review
- Transcriptome analysis of genes related to glucoraphanin and sulforaphane synthesis in methyl jasmonate treated broccoli (Brassica oleracea var. italica) hairy roots.Journal of plant research · 2022Article
- Review
- Functional Characterization of UDP-Glycosyltransferases Involved in Anti-viral Lignan Glycosides Biosynthesis inFrontiers in plant science · 2022Article
- Article
- Isatis indigotica: from (ethno) botany, biochemistry to synthetic biology.Molecular horticulture · 2021Review
- Water stress intensified the relation of seed color with lignan content and seed yield components in flax (Linum usitatissimum L.).Scientific reports · 2021Article
- Lignans of Sesame (Molecules (Basel, Switzerland) · 2021Review
- The Role of Polyphenols in Abiotic Stress Response: The Influence of Molecular Structure.Plants (Basel, Switzerland) · 2021Review
- Full-Length Transcriptome Analyses of Genes Involved in Triterpenoid Saponin Biosynthesis ofFrontiers in genetics · 2021Article
- Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes.Plant molecular biology · 2020Article
- Enhanced Biosynthesis of Chlorogenic Acid and Its Derivatives in Methyl-Jasmonate-TreatedFrontiers in bioengineering and biotechnology · 2020Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, and are widely used as "Banlangen" and "Daqingye" in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group of lignan metabolites. However, the biosynthesis of lignans in I. indigotica remains opaque. This study describes the discovery and analysis of biosynthetic genes and AP2/ERF-type transcription factors involved in lignan biosynthesis in I. indigotica. MeJA treatment revealed differential expression of three genes involved in phenylpropanoid backbone biosynthesis (IiPAL, IiC4H, Ii4CL), five genes involved in lignan biosynthesis (IiCAD, IiC3H, IiCCR, IiDIR, and IiPLR), and 112 putative AP2/ERF transcription factors. In addition, four intermediates of lariciresinol biosynthesis were found to be induced. Based on these results, a canonical correlation analysis using Pearson's correlation coefficient was performed to construct gene-to-metabolite networks and identify putative key genes and rate-limiting reactions in lignan biosynthesis. Over-expression of IiC3H, identified as a key pathway gene, was used for metabolic engineering of I. indigotica hairy roots, and resulted in an increase in lariciresinol production. These findings illustrate the utility of canonical correlation analysis for the discovery and metabolic engineering of key metabolic genes in plants.
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