Evidence map›Paper›PMID 26579166›Full record

ArticleFrontiers in plant science2015

Yeast functional screen to identify genes conferring salt stress tolerance in Salicornia europaea.

Yoshiki Nakahara, Shogo Sawabe, Kenta Kainuma, Maki Katsuhara, Mineo Shibasaka, Masanori Suzuki, Kosuke Yamamoto, Suguru Oguri, Hikaru Sakamoto

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Yoshiki NakaharaInstitute of Plant Science and Resources, Okayama University Kurashiki, Japan.
Shogo SawabeGraduate School of Biological Sciences, Nara Institute of Science and Technology Ikoma, Japan.
Kenta KainumaFaculty of Bioindustry, Tokyo University of Agriculture Abashiri, Japan.
Maki KatsuharaInstitute of Plant Science and Resources, Okayama University Kurashiki, Japan.
Mineo ShibasakaInstitute of Plant Science and Resources, Okayama University Kurashiki, Japan.
Masanori SuzukiFaculty of Bioindustry, Tokyo University of Agriculture Abashiri, Japan.
Kosuke YamamotoAdvanced Life Science Institute Inc. Wako, Japan.
Suguru OguriFaculty of Bioindustry, Tokyo University of Agriculture Abashiri, Japan.
Hikaru SakamotoFaculty of Bioindustry, Tokyo University of Agriculture Abashiri, Japan.
Tokyo University of Agriculture · JPOkayama University · JPAdvanced Life Science Institute · JPNara Institute of Science and Technology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Salinity is a critical environmental factor that adversely affects crop productivity. Halophytes have evolved various mechanisms to adapt to saline environments. Salicornia europaea L. is one of the most salt-tolerant plant species. It does not have special salt-secreting structures like a salt gland or salt bladder, and is therefore a good model for studying the common mechanisms underlying plant salt tolerance. To identify candidate genes encoding key proteins in the mediation of salt tolerance in S. europaea, we performed a functional screen of a cDNA library in yeast. The library was screened for genes that allowed the yeast to grow in the presence of 1.3 M NaCl. We obtained three full-length S. europaea genes that confer salt tolerance. The genes are predicted to encode (1) a novel protein highly homologous to thaumatin-like proteins, (2) a novel coiled-coil protein of unknown function, and (3) a novel short peptide of 32 residues. Exogenous application of a synthetic peptide corresponding to the 32 residues improved salt tolerance of Arabidopsis. The approach described in this report provides a rapid assay system for large-scale screening of S. europaea genes involved in salt stress tolerance and supports the identification of genes responsible for such mechanisms. These genes may be useful candidates for improving crop salt tolerance by genetic transformation.

Indexed as

coiled-coil proteinSalicornia europaea L.salt stressshort peptidethaumatin-like proteinyeast functional screen

Identifiers

PMID26579166
PMCPMC4623525
OpenAlexW2177932567

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.