ArticleFrontiers in microbiology2015
Current and future resources for functional metagenomics.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
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Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Recent Advanced Technologies for the Characterization of Xenobiotic-Degrading Microorganisms and Microbial Communities.Frontiers in bioengineering and biotechnology · 2021Pooled it
- Microbial lipases: advances in metagenomics and artificial intelligence for enzyme discovery and engineering.Archives of microbiology · 2026Review
- Deep Learning-Enhanced Raman Microspectroscopy Enables Rapid Microbial Classification and Captures Phylogenetic Relationships.Microorganisms · 2026Article
- Metagenomic libraries data sets from the hypersaline benthic microbial mats of the Fraternidad Lagoon, Puerto Rico, using an indirect DNA extraction method.Microbiology resource announcements · 2026Article
- A comparison of routine blood culture methods and multiplex quantitative PCR for detecting pathogens in simulated polymicrobial blood cultures.Journal of medical microbiology · 2026Article
- Insects as a New Source of Plant Growth Promoting Bacteria - Review.Microbial ecology · 2026Review
- Geographics and bacterial networks differently shape the acquired and latent global sewage resistomes.Nature communications · 2025Article
- Article
- Unearthing the genetic resources of Arabian sea seamount and metagenomic insights into phosphate cycling genes for next generation plant biostimulants.Scientific reports · 2025Article
- A Microfluidics-Based Ultrahigh-Throughput Screening Unveils Diverse Ketoreductases Relevant to Pharmaceutical Synthesis.Analytical chemistry · 2025Article
- Review
- Genomic insights into novel extremotolerant bacteria isolated from the NASA Phoenix mission spacecraft assembly cleanrooms.Microbiome · 2025Article
- A non-hypothesis-driven practical laboratory activity on functional metagenomics: "fishing" protein-coding DNA sequences from microbiomes.Frontiers in bioengineering and biotechnology · 2025Article
- Construction and characterization of DNA libraries from cultured phages and environmental viromes.Applied and environmental microbiology · 2024Article
- High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies.Applied and environmental microbiology · 2024Article
- Environmental activity-based protein profiling for function-driven enzyme discovery from natural communities.Environmental microbiome · 2024Article
- Metagenomic exploration of cold-active enzymes for detergent applications: Characterization of a novel, cold-active and alkali-stable GH8 endoglucanase from ikaite columns in SW Greenland.Microbial biotechnology · 2024Article
- A novel GH3-β-glucosidase from soda lake metagenomic libraries with desirable properties for biomass degradation.Scientific reports · 2024Article
- Metabolites: a converging node of host and microbe to explain meta-organism.Frontiers in microbiology · 2024Review
- Microbial community diversity of an integrated constructed wetland used for treatment of sewage.Frontiers in microbiology · 2024Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional metagenomics is a powerful experimental approach for studying gene function, starting from the extracted DNA of mixed microbial populations. A functional approach relies on the construction and screening of metagenomic libraries-physical libraries that contain DNA cloned from environmental metagenomes. The information obtained from functional metagenomics can help in future annotations of gene function and serve as a complement to sequence-based metagenomics. In this Perspective, we begin by summarizing the technical challenges of constructing metagenomic libraries and emphasize their value as resources. We then discuss libraries constructed using the popular cloning vector, pCC1FOS, and highlight the strengths and shortcomings of this system, alongside possible strategies to maximize existing pCC1FOS-based libraries by screening in diverse hosts. Finally, we discuss the known bias of libraries constructed from human gut and marine water samples, present results that suggest bias may also occur for soil libraries, and consider factors that bias metagenomic libraries in general. We anticipate that discussion of current resources and limitations will advance tools and technologies for functional metagenomics research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.