ArticleProceedings of the National Academy of Sciences of the United States of America2015
Pentatricopeptide-repeat family protein RF6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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94 citing papers in PubMed, 190 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Non-functional allele ofPlant biotechnology (Tokyo, Japan) · 2026Article
- A distorter-restorer system drives quantitative reproductive isolation in rice.Nature plants · 2026Article
- A PPR gene cluster from Solanum aethiopicum underlies fertility restoration in cytoplasmic male-sterile eggplant (S. melongena L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Two nuclear loci Rf-m1 and Rf-m2 interplay to determine the fertility restoring efficacy of ZD-type CMS soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Molecular and physiological basis of heterosis in hybrid rice performance.Molecular breeding : new strategies in plant improvement · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.Plant cell reports · 2025Review
- Orf116b Induces Pollen Abortion in a Novel Cotton (International journal of molecular sciences · 2024Article
- Review
- A P-type pentatricopeptide repeat protein ZmRF5 promotes 5' region partial cleavages of atp6c transcripts to restore the fertility of CMS-C maize by recruiting a splicing factor.Plant biotechnology journal · 2024Article
- Mitochondrial atp1 mRNA knockdown by a custom-designed pentatricopeptide repeat protein alters ATP synthase.Plant physiology · 2024Article
- PPR proteins in plants: roles, mechanisms, and prospects for rice research.Frontiers in plant science · 2024Review
- Establishment of new convenient two-line system for hybrid production by targeting mutation ofHorticulture research · 2023Article
- Mapping of Rf20(t), a minor fertility restorer gene for rice wild abortive cytoplasmic male sterility in the maintainer line 'Zhenshan97B'.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Dose effects of restorer gene modulate pollen fertility in cotton CMS-D2 restorer lines via auxin signaling and flavonoid biosynthesis.Plant cell reports · 2023Article
- Fine mapping and candidate gene analysis of a novel fertility restorer gene for C-type cytoplasmic male sterility in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic Analysis of Novel Fertility Restoration Genes (International journal of molecular sciences · 2023Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) has been extensively used for hybrid seed production in many major crops. Honglian CMS (HL-CMS) is one of the three major types of CMS in rice and has contributed greatly to food security worldwide. The HL-CMS trait is associated with an aberrant chimeric mitochondrial transcript, atp6-orfH79, which causes pollen sterility and can be rescued by two nonallelic restorer-of-fertility (Rf) genes, Rf5 or Rf6. Here, we report the identification of Rf6, which encodes a novel pentatricopeptide repeat (PPR) family protein with a characteristic duplication of PPR motifs 3-5. RF6 is targeted to mitochondria, where it physically associates with hexokinase 6 (OsHXK6) and promotes the processing of the aberrant CMS-associated transcript atp6-orfH79 at nucleotide 1238, which ensures normal pollen development and restores fertility. The duplicated motif 3 of RF6 is essential for RF6-OsHXK6 interactions, processing of the aberrant transcript, and restoration of fertility. Furthermore, reductions in the level of OsHXK6 result in atp6-orfH79 transcript accumulation and male sterility. Together these results reveal a novel mechanism for CMS restoration by which RF6 functions with OsHXK6 to restore HL-CMS fertility. The present study also provides insight into the function of hexokinase 6 in regulating mitochondrial RNA metabolism and may facilitate further exploitation of heterosis in rice.
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