ArticleNucleic acids research2016
An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Transcription-replication collisions trigger high-fidelity replication reset.Nucleic acids research · 2025Article
- GoldenBraid2.0Synthetic biology (Oxford, England) · 2025Article
- The F plasmid conjutome: the repertoire ofmSphere · 2024Article
- Drugging evolution of antibiotic resistance at a regulatory network hub.Science advances · 2023Article
- ppGpp and RNA-polymerase backtracking guide antibiotic-induced mutable gambler cells.Molecular cell · 2023Article
- Stress-Induced Mutagenesis, Gambler Cells, and Stealth Targeting Antibiotic-Induced Evolution.mBio · 2022Review
- Evolutionary action of mutations reveals antimicrobial resistance genes in Escherichia coli.Nature communications · 2022Article
- Article
- Gamblers: An Antibiotic-Induced Evolvable Cell Subpopulation Differentiated by Reactive-Oxygen-Induced General Stress Response.Molecular cell · 2019Article
- Article
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- Directed evolution of Escherichia coli with lower-than-natural plasmid mutation rates.Nucleic acids research · 2018Article
- CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.Nature communications · 2018Article
- Persistent damaged bases in DNA allow mutagenic break repair in Escherichia coli.PLoS genetics · 2017Article
- The Small RNA GcvB Promotes Mutagenic Break Repair by Opposing the Membrane Stress Response.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis, screens, and WGS in Escherichia coli. We created Deconvoluter-ordered libraries with selectable insertions every 50 kb in the E. coli genome. The Deconvoluter method uses these for replacement of untagged mutations in the genome using a phage-P1-based gene-replacement strategy. We validate the Deconvoluter resource by deconvolution of 17 of 17 phenotype-altering mutations from a screen of N-ethyl-N-nitrosourea-induced mutants. The Deconvoluter resource permits rapid unbiased screens and gene/function identification and will enable exploration of functions of essential genes and undiscovered genes/sites/alleles not represented in existing deletion collections. This resource for unbiased forward-genetic screens with mapping-by-sequencing ('forward genomics') demonstrates a strategy that could similarly enable rapid screens in many other microbes.
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