ArticleBiomedical chromatography : BMC2016
An HPLC method with diode array detector for the simultaneous quantification of chloroquine and desethylchloroquine in plasma and whole blood samples from Plasmodium vivax patients in Vietnam, using quinine as an internal standard.
Article in Biomedical chromatography : BMC, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 13 citations in OpenAlex.
- Influence of CYP2C8 Polymorphism on the Exposure to Chloroquine in Patients with Malaria byInternational journal of environmental research and public health · 2025Article
- Analytical approaches for determination of COVID-19 candidate drugs in human biological matrices.Trends in analytical chemistry : TRAC · 2023Review
- Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine.Journal of chromatographic science · 2021Review
- HPLC methods for choloroquine determination in biological samples and pharmaceutical products.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2021Review
- Absence of gender influence on the pharmacokinetics of chloroquine combined with primaquine in malaria vivax patients.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Doses of chloroquine in the treatment of malaria by Plasmodium vivax in patients between 2 and 14 years of age from the Brazilian Amazon basin.Malaria journal · 2019Article
- High sensitivity methods to quantify chloroquine and its metabolite in human blood samples using LC-MS/MS.Bioanalysis · 2019Article
- An HPLC method with diode array detector for the simultaneous quantification of chloroquine and desethylchloroquine in plasma and whole blood samples from Plasmodium vivax patients in Vietnam, using quinine as an internal standard.Biomedical chromatography : BMC · 2016Article
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9 authors at 4 institutions in 4 countries.
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Abstract
A sensitive, simple method for quantification of chloroquine (CQ) and desethylchloroquine (MCQ) in whole blood and plasma from Plasmodium vivax patients has been developed using HPLC with diode array detection (DAD). Solid-phase extraction on Isolute-96-CBA was employed to process 100 μL of plasma/whole blood samples. CQ, MCQ and quinine were separated using a mobile phase of phosphate buffer 25 mm, pH 2.60-acetonitrile (88:12, v/v) with 2 mm sodium perchlorate on a Zorbax SB-CN 150 × 4.6 mm, 5 μm column at a flow rate of 1.2 mL/min, at ambient temperature in 10 min, with the DAD wavelength of 343 nm. The method was linear over the range of 10-5000 ng/mL for both CQ and MCQ in plasma and whole blood. The limit of detection was 4 ng/mL and limit of quantification was 10 ng/mL in both plasma and blood for CQ and MCQ. The intra-, inter- and total assay precision were <10% for CQ and MCQ in plasma and whole blood. In plasma, the accuracies varied between 101 and 103%, whereas in whole blood, the accuracies ranged from 97.0 to 102% for CQ and MCQ. The method is an ideal technique with simple facilities and instruments, bringing about good separation in comparison with previous methods. © 2016 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd.
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