Evidence map›Paper›PMID 26578091›Full record

ArticleScientific reports2015

Molecular cloning and characterization of amh and dax1 genes and their expression during sex inversion in rice-field eel Monopterus albus.

Qing Hu, Wei Guo, Yu Gao, Rong Tang, Dapeng Li

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 48 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Qing HuCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Wei GuoCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Yu GaoCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Rong TangCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Dapeng LiCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Ministry of Agriculture and Rural Affairs · CNHuazhong Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The full-length cDNAs of amh and dax1 in the hermaphrodite, rice-field eel (Monopterus albus), were cloned and characterized in this study. Multiple sequence alignment revealed Dax1 was well conserved among vertebrates, whereas Amh had a low degree of similarity between different vertebrates. Their expression profiles in gonads during the course of sex inversion and tissues were investigated. The tissue distribution indicated amh was expressed mostly in gonads and was scarcely detectable in other tissues, whereas the expression of dax1 was widespread among the different tissues, especially liver and gonads. amh was scarcely detectable in ovaries whereas it was abundantly expressed in both ovotestis and testis. By contrast, dax1 was highly expressed in ovaries, especially in ♀IV (ovaries in IV stage), but it was decreased significantly in ♀/♂I (ovotestis in I stage). Its expression was increased again in ♀/♂III (ovotestis in III stage), and then decreased to a low level in testis. These significant different expression patterns of amh and dax1 suggest the increase of amh expression and the decline of dax1 expression are important for the activation of testis development, and the high level of amh and a low level of dax1 expression are necessary for maintenance of testis function.

Indexed as

Cloning, MolecularGene ExpressionAmino Acid SequenceAnimalsAnti-Mullerian HormoneBase SequenceDAX-1 Orphan Nuclear ReceptorFemaleFishesMaleMolecular Sequence DataOrgan SpecificityPhylogenySequence AlignmentSequence Analysis, DNASex DifferentiationAnti-Mullerian HormoneDAX-1 Orphan Nuclear Receptor

Identifiers

PMID26578091
PMCPMC4649613
OpenAlexW2279790355

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.