ArticleScientific reports2015
The AP-3 adaptor complex mediates sorting of yeast and mammalian PQ-loop-family basic amino acid transporters to the vacuolar/lysosomal membrane.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Using CMAC Staining for Vacuole Characterization in Yeast.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Loss of the HOPS complex disrupts early-to-late endosome transition, impairs endosomal recycling and induces accumulation of amphisomes.Molecular biology of the cell · 2024Article
- The yeast endocytic early/sorting compartment exists as an independent sub-compartment within theeLife · 2023Article
- Overlapping Machinery in Lysosome-Related Organelle Trafficking: A Lesson from Rare Multisystem Disorders.Cells · 2022Review
- Three-Dimensional Genome Map of the Filamentous FungusMicrobiology spectrum · 2022Article
- Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization.Cell reports · 2022Article
- The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.Autophagy · 2022Article
- Role of adaptin protein complexes in intracellular trafficking and their impact on diseases.Bioengineered · 2021Article
- Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acids.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Nitrogen coordinated import and export of arginine across the yeast vacuolar membrane.PLoS genetics · 2020Article
- Article
- The road to lysosome-related organelles: Insights from Hermansky-Pudlak syndrome and other rare diseases.Traffic (Copenhagen, Denmark) · 2019Review
- A systematic approach to identify recycling endocytic cargo depending on the GARP complex.eLife · 2019Article
- A Kinetic View of Membrane Traffic Pathways Can Transcend the Classical View of Golgi Compartments.Frontiers in cell and developmental biology · 2019Review
- Amino Acid Metabolism and Transport Mechanisms as Potential Antifungal Targets.International journal of molecular sciences · 2018Review
- A Genetic Screen for Investigating the Human Lysosomal CystineTransporter, Cystinosin.Scientific reports · 2018Article
- Article
- Budding Yeast Has a Minimal Endomembrane System.Developmental cell · 2018Article
- A genome-wide copper-sensitized screen identifies novel regulators of mitochondrial cytochrome c oxidase activity.The Journal of biological chemistryArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The limiting membrane of lysosomes in animal cells and that of the vacuole in yeast include a wide variety of transporters, but little is known about how these proteins reach their destination membrane. The mammalian PQLC2 protein catalyzes efflux of basic amino acids from the lysosome, and the similar Ypq1, -2, and -3 proteins of yeast perform an equivalent function at the vacuole. We here show that the Ypq proteins are delivered to the vacuolar membrane via the alkaline phosphatase (ALP) trafficking pathway, which requires the AP-3 adaptor complex. When traffic via this pathway is deficient, the Ypq proteins pass through endosomes from where Ypq1 and Ypq2 properly reach the vacuolar membrane whereas Ypq3 is missorted to the vacuolar lumen via the multivesicular body pathway. When produced in yeast, PQLC2 also reaches the vacuolar membrane via the ALP pathway, but tends to sort to the vacuolar lumen if AP-3 is defective. Finally, in HeLa cells, inhibiting the synthesis of an AP-3 subunit also impairs sorting of PQLC2 to lysosomes. Our results suggest the existence of a conserved AP-3-dependent trafficking pathway for proper delivery of basic amino acid exporters to the yeast vacuole and to lysosomes of human cells.
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