ArticleDevelopmental biology2016
Cell cycle features of C. elegans germline stem/progenitor cells vary temporally and spatially.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- Basal cytoplasmic calcium levels regulate C. elegans germ stem cell proliferation.Development (Cambridge, England) · 2026Article
- Histone Modifications in the Cell Cycle ofEpigenomes · 2026Review
- Genetic mutations in GLP-1/Notch pathway reveal distinct mechanisms of Notch signaling in germline stem cell regulation.Biology open · 2026Article
- Pre-dauer starvation rapidly and reversibly reduces niche proliferative signaling to the Caenorhabditis elegans germ line.Development (Cambridge, England) · 2026Article
- Comprehensive comparative analysis of the effects of temperature on the Notch signaling response in vivo.Biology open · 2025Article
- Non-autonomy of age-related morphological changes in the C. elegans germline stem cell niche.Development (Cambridge, England) · 2025Article
- Non-autonomy of age-related morphological changes in thebioRxiv : the preprint server for biology · 2025Article
- An RNAi screen of Rab GTPase genes in Caenorhabditis elegans reveals that morphogenesis has a higher demand than stem cell niche maintenance for rab-1 in the somatic cells of the reproductive system.G3 (Bethesda, Md.) · 2025Article
- Review
- Intestinal RICT-1 regulates the larval germline progenitor pool via the vitellogenin VIT-3 inbioRxiv : the preprint server for biology · 2025Article
- Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells.PLoS genetics · 2024Article
- The roles of TGFβ and serotonin signaling in regulating proliferation of oocyte precursors and germline aging.bioRxiv : the preprint server for biology · 2024Article
- Aging disrupts spatiotemporal regulation of germline stem cells and niche integrity.Biology open · 2024Article
- Germline mutation rate is elevated in young and old parents inEvolution letters · 2023Article
- The CERV protein of Cer1, a C. elegans LTR retrotransposon, is required for nuclear export of viral genomic RNA and can form giant nuclear rods.PLoS genetics · 2023Article
- The serotonin circuit that coordinates germline proliferation and egg laying with other reproductive functions inProceedings. Biological sciences · 2022Article
- A male pheromone that improves the quality of the oogenic germline.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Molecular basis of reproductive senescence: insights from model organisms.Journal of assisted reproduction and genetics · 2021Review
- Germline Stem and Progenitor Cell Aging inFrontiers in cell and developmental biology · 2021Review
- Starvation Responses Throughout theGenetics · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Many organisms accumulate a pool of germline stem cells during development that is maintained in later life. The dynamics of establishment, expansion and homeostatic maintenance of this pool are subject to both developmental and physiological influences including the availability of a suitable niche microenvironment, nutritional status, and age. Here, we investigated the dynamics of germline proliferation during stages of expansion and homeostasis, using the C. elegans germ line as a model. The vast majority of germ cells in the proliferative zone are in interphase stages of mitosis (G1, S, G2) rather than in the active mitotic (M) phase. We examined mitotic index and DNA content, comparing different life stages, mutants, and physiological conditions. We found that germ cells in larval stages cycle faster than in adult stages, but that this difference could not be attributed to sexual fate of the germ cells. We also found that larval germ cells exhibit a lower average DNA content compared to adult germ cells. We extended our analysis to consider the effects of distance from the niche and further found that the spatial pattern of DNA content differs between larval and adult stages in the wild type and among mutants in pathways that interfere with cell cycle progression, cell fate, or both. Finally, we characterized expansion of the proliferative pool of germ cells during adulthood, using a regeneration paradigm (ARD recovery) in which animals are starved and re-fed. We compared adult stage regeneration and larval stage expansion, and found that the adult germ line is capable of rapid accumulation but does not sustain a larval-level mitotic index nor does it recapitulate the larval pattern of DNA content. The regenerated germ line does not reach the number of proliferative zone nuclei seen in the continuously fed adult. Taken together, our results suggest that cell cycle dynamics are under multiple influences including distance from the niche, age and/or maturation of the germ line, nutrition and, possibly, latitude for physical expansion.
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