ArticleProceedings of the National Academy of Sciences of the United States of America1989
Rapid turnover of microtubule-associated protein MAP2 in the axon revealed by microinjection of biotinylated MAP2 into cultured neurons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 61 citations in OpenAlex.
- Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons.The Journal of biological chemistry · 2026Article
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- Ischemic tolerance in an in vivo model of glutamate preconditioning.Journal of neuroscience research · 2015Article
- Tau and axonopathy in neurodegenerative disorders.Neuromolecular medicine · 2002Review
- Novel dendritic kinesin sorting identified by different process targeting of two related kinesins: KIF21A and KIF21B.The Journal of cell biology · 1999Article
- Making sense of the multiple MAP-2 transcripts and their role in the neuron.Molecular neurobiology · 1998Review
- Tau binds to the distal axon early in development of polarity in a microtubule- and microfilament-dependent manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Effect of melatonin on beta-tubulin and MAP2 expression in NIE-115 cells.Neurochemical research · 1996Article
- Selective stabilization of tau in axons and microtubule-associated protein 2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons.The Journal of cell biology · 1996Article
- A neuron-specific isoform of brain ankyrin, 440-kD ankyrinB, is targeted to the axons of rat cerebellar neurons.The Journal of cell biology · 1995Article
- KIF3A is a new microtubule-based anterograde motor in the nerve axon.The Journal of cell biology · 1994Article
- Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin.Journal of protein chemistry · 1994Article
- Incorporation and turnover of biotin-labeled actin microinjected into fibroblastic cells: an immunoelectron microscopic study.The Journal of cell biology · 1989Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
We studied the mechanism of compartmentation of microtubule-associated protein 2 (MAP2) in the dendrites and cell bodies by using microinjection of biotin-labeled MAP2 into mature spinal cord neurons in culture. MAP2 molecules microinjected into the nerve cell body were distributed not only throughout the cytoplasm of the cell body and dendrites, but also in the axon as far as a few millimeters from the cell body within 24 hr after injection. However, when injected cells were incubated for more than 3 days, the amount of biotin-labeled MAP2 in the axon decreased remarkably compared with that in the dendrites. This indicates that there is no sorting mechanism in the cell body for the transport of MAP2 selectively into the dendrites but that the turnover rate of MAP2 in the axons differs from that in the dendrites. To further characterize the mechanism of MAP2 compartmentation, we performed immunoelectron microscopy of injected cells and detergent extraction of microinjected cells prior to immunocytochemistry with anti-biotin. The results strongly suggest that a large part of axonal MAP2 is not associated with cytoskeleton and that this weak association of MAP2 favors selective loss of MAP2 from the axon.
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