ArticleJournal of molecular evolution2015
Identification of the Same Na(+)-Specific DNAzyme Motif from Two In Vitro Selections Under Different Conditions.
Article in Journal of molecular evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- One-step electrochemical aptasensor for lactate detection in athlete training using AuPt NPs and PEI-rGO composite with NaRSC advances · 2025Article
- Simultaneous FeScience advances · 2023Article
- DNAzymes as Activity-Based Sensors for Metal Ions: Recent Applications, Demonstrated Advantages, Current Challenges, and Future Directions.Accounts of chemical research · 2019Review
- From general base to general acid catalysis in a sodium-specific DNAzyme by a guanine-to-adenine mutation.Nucleic acids research · 2019Article
- Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium.Nucleic acids research · 2018Article
- Theranostic DNAzymes.Theranostics · 2017Review
- A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing.Nucleic acids research · 2016Article
- Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes.Inorganica chimica acta · 2016Article
- Improving the odds: Influence of starting pools on in vitro selection outcomes.Methods (San Diego, Calif.) · 2016Review
- A DNAzyme requiring two different metal ions at two distinct sites.Nucleic acids research · 2016Article
- A Quarter Century of In Vitro Selection.Journal of molecular evolution · 2015Article
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2 authors.
Funding
Abstract
We report an investigation of the functional relationship between two independently selected RNA-cleaving DNAzymes, NaA43, and Ce13, through in vitro selection. The NaA43 DNAzyme was obtained through a combination of gel-based and column-based in vitro selection in the presence of Na(+) and reported to be highly selective for Na(+) over other metal ions. The Ce13 DNAzyme was isolated via a gel-based method in the presence of Ce(4+) and found to be active with trivalent lanthanides, Y(3+) and Pb(2+). Despite completely different activities reported for the two DNAzymes, they share a high level of sequence similarity (~60% sequence identity). In this work, we systematically analyzed the activity of both DNAzymes to elucidate their potential functional relationship. We found that Na(+) is an obligate cofactor of the Ce13 DNAzyme and lanthanides cannot initiate the cleavage reaction in the absence of Na(+). Hence, we conclude that the Ce13 DNAzyme is a variant of the NaA43 DNAzyme that catalyzes reaction in the presence Na(+) and also utilizes lanthanides in a potentially allosteric manner. These results have identified a new DNAzyme motif that is not only remarkably Na(+)-specific, but also allows for design of novel allosteric DNAzymes for different biotechnological applications.
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