ArticleScientific reports2015
High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system.Cell research · 2024Article
- Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis.International journal of molecular sciences · 2023Review
- Active image optimization for lattice light sheet microscopy in thick samples.Biomedical optics express · 2022Article
- Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus.Frontiers in cell and developmental biology · 2021Article
- Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies.Frontiers in cell and developmental biology · 2021Article
- Influence ofInternational journal of molecular sciences · 2020Article
- Imaging of the immune system - towards a subcellular and molecular understanding.Journal of cell science · 2020Review
- Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward.Frontiers in molecular biosciences · 2020Article
- Highly Sensitive Shack-Hartmann Wavefront Sensor: Application to Non-Transparent Tissue Mimic Imaging with Adaptive Light-Sheet Fluorescence Microscopy.Methods and protocols · 2019Article
- Multiscale light-sheet for rapid imaging of cardiopulmonary system.JCI insight · 2018Review
- Light-sheet microscopy with attenuation-compensated propagation-invariant beams.Science advances · 2018Article
- Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM.Light, science & applications · 2018Article
- Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy.Nature communications · 2017Article
- Deciphering tissue morphodynamics using bioimage informatics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- STED imaging performance estimation by means of Fourier transform analysis.Biomedical optics express · 2017Article
- A guide to light-sheet fluorescence microscopy for multiscale imaging.Nature methods · 2017Review
- Imaging tissue-mimic with light sheet microscopy: A comparative guideline.Scientific reports · 2017Article
- Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms.Nature biotechnology · 2016Article
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Authors and funding
6 authors.
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Abstract
Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids.
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