ArticlePlant physiology2016
Profilin-Dependent Nucleation and Assembly of Actin Filaments Controls Cell Elongation in Arabidopsis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Unconventional vesicle transport driven by formin-actin-myosin working system during pollen germination.Science advances · 2026Article
- Agent-Based Computational Modeling of the Stochastic Dynamic Behavior of Actin Filaments Recapitulates the Homeostatic Cortical Array in Plant Epidermal Cells.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Please inhibit responsibly: Natural and synthetic actin toxins as useful tools in cell biology.Molecular biology of the cell · 2025Review
- OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility.Plant communications · 2025Article
- Regulation of cytoskeleton dynamics and its interplay with force in plant cells.Biophysics reviews · 2024Review
- Cooperative actin filament nucleation by the Arp2/3 complex and formins maintains the homeostatic cortical array in Arabidopsis epidermal cells.The Plant cell · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Transcriptomic Analysis of Radish (Plants (Basel, Switzerland) · 2022Article
- Alterations to the broad-spectrum formin inhibitor SMIFH2 modulate potency but not specificity.Scientific reports · 2022Article
- Lipid Signaling Requires ROS Production to Elicit Actin Cytoskeleton Remodeling during Plant Innate Immunity.International journal of molecular sciences · 2022Article
- Article
- The 5-formyl-tetrahydrofolate proteome links folates with C/N metabolism and reveals feedback regulation of folate biosynthesis.The Plant cell · 2021Article
- Formins control dynamics of F-actin in the central cell ofPlant signaling & behavior · 2021Article
- Analysis of formin functions during cytokinesis using specific inhibitor SMIFH2.Plant physiology · 2021Article
- Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth.Frontiers in plant science · 2021Review
- Auxin-induced actin cytoskeleton rearrangements require AUX1.The New phytologist · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-inFrontiers in plant science · 2020Article
- Proteomic analysis of a clavata-like phenotype mutant in Brassica napus.Genetics and molecular biology · 2020Article
- Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin filaments in plant cells are incredibly dynamic; they undergo incessant remodeling and assembly or disassembly within seconds. These dynamic events are choreographed by a plethora of actin-binding proteins, but the exact mechanisms are poorly understood. Here, we dissect the contribution of Arabidopsis (Arabidopsis thaliana) PROFILIN1 (PRF1), a conserved actin monomer-binding protein, to actin organization and single filament dynamics during axial cell expansion of living epidermal cells. We found that reduced PRF1 levels enhanced cell and organ growth. Surprisingly, we observed that the overall frequency of nucleation events in prf1 mutants was dramatically decreased and that a subpopulation of actin filaments that assemble at high rates was reduced. To test whether profilin cooperates with plant formin proteins to execute actin nucleation and rapid filament elongation in cells, we used a pharmacological approach. Here, we used Small Molecule Inhibitor of Formin FH2 (SMIFH2), after validating its mode of action on a plant formin in vitro, and observed a reduced nucleation frequency of actin filaments in live cells. Treatment of wild-type epidermal cells with SMIFH2 mimicked the phenotype of prf1 mutants, and the nucleation frequency in prf1-2 mutant was completely insensitive to these treatments. Our data provide compelling evidence that PRF1 coordinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucleation and assembly during plant cell expansion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.