ArticleMicrobial ecology2016
Expansion of Cultured Bacterial Diversity by Large-Scale Dilution-to-Extinction Culturing from a Single Seawater Sample.
Article in Microbial ecology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Novel roseophages provide insights into a genetically and ecologically diverse phage family.Microbial genomics · 2025Article
- Microbial metagenomes from Lake Soyang, the largest freshwater reservoir in South Korea.Scientific data · 2025Article
- Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.Marine drugs · 2025Review
- New isolates refine the ecophysiology of the Roseobacter CHAB-I-5 lineage.ISME communications · 2025Article
- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- Metagenomic data from surface seawater of the east coast of South Korea.Scientific data · 2023Article
- Top abundant deep ocean heterotrophic bacteria can be retrieved by cultivation.ISME communications · 2023Article
- Article
- Accessing previously uncultured marine microbial resources by a combination of alternative cultivation methods.Microbial biotechnology · 2021Article
- Highly Contaminated Marine Sediments Can Host Rare Bacterial Taxa Potentially Useful for Bioremediation.Frontiers in microbiology · 2021Article
- Using Plate-Wash PCR and High-Throughput Sequencing to Measure Cultivated Diversity for Natural Product Discovery Efforts.Frontiers in microbiology · 2021Article
- High-throughput cultivation based on dilution-to-extinction with catalase supplementation and a case study of cultivating acI bacteria from Lake Soyang.Journal of microbiology (Seoul, Korea) · 2020Article
- Expanding the Diversity of Bacterioplankton Isolates and Modeling Isolation Efficacy with Large-Scale Dilution-to-Extinction Cultivation.Applied and environmental microbiology · 2020Article
- Diversity and distribution of marine heterotrophic bacteria from a large culture collection.BMC microbiology · 2020Article
- Exploration of the key functional strains from an azo dye degradation microbial community by DGGE and high-throughput sequencing technology.Environmental science and pollution research international · 2019Article
- The CURE for Cultivating Fastidious Microbes.Journal of microbiology & biology education · 2019Article
- Putative Mixotrophic Nitrifying-Denitrifying Gammaproteobacteria Implicated in Nitrogen Cycling Within the Ammonia/Oxygen Transition Zone of an Oil Sands Pit Lake.Frontiers in microbiology · 2019Article
- Study of Bacterial Community Composition and Correlation of Environmental Variables in Rambla Salada, a Hypersaline Environment in South-Eastern Spain.Frontiers in microbiology · 2018Article
- Verrucomicrobia are prevalent in north-temperate freshwater lakes and display class-level preferences between lake habitats.PloS one · 2018Article
- The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures.Scientific reports · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput cultivation (HTC) based on a dilution-to-extinction method has been applied broadly to the cultivation of marine bacterial groups, which has often led to the repeated isolation of abundant lineages such as SAR11 and oligotrophic marine gammaproteobacteria (OMG). In this study, to expand the phylogenetic diversity of HTC isolates, we performed a large-scale HTC with a single surface seawater sample collected from the East Sea, the Western Pacific Ocean. Phylogenetic analyses of the 16S rRNA genes from 847 putative pure cultures demonstrated that some isolates were affiliated with not-yet-cultured clades, including the OPB35 and Puniceicoccaceae marine group of Verrucomicrobia and PS1 of Alphaproteobacteria. In addition, numerous strains were obtained from abundant clades, such as SAR11, marine Roseobacter clade, OMG (e.g., SAR92 and OM60), OM43, and SAR116, thereby increasing the size of available culture resources for representative marine bacterial groups. Comparison between the composition of HTC isolates and the bacterial community structure of the seawater sample used for HTC showed that diverse marine bacterial groups exhibited various growth capabilities under our HTC conditions. The growth response of many bacterial groups, however, was clearly different from that observed with conventional plating methods, as exemplified by numerous isolates of the SAR11 clade and Verrucomicrobia. This study showed that a large number of novel bacterial strains could be obtained by an extensive HTC from even a small number of samples.
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